biologia plantarum

Biologia plantarum 52:517-520, 2008 | DOI: 10.1007/s10535-008-0100-x

In vitro propagation of Capsicum chinense Jacq.

K. Sanatombi¹, G. J. Sharma^1,*

¹ Department of Life Sciences, Manipur University, Imphal, India

An efficient micropropagation protocol was established for Capsicum chinense Jacq. cv. Umorok, a pungent chilli cultivar. Shoot-tip explants were cultured on Murashige and Skoog (MS) medium containing cytokinins (22.2-88.8 µM 6-benzylaminopurine, BAP, 23.2-93.0 µM kinetin, Kin, or 22.8-91.2 µM zeatin, Z) alone or in combination with 5.7 µM indole-3-acetic acid (IAA). Maximum number of shoots were induced on medium containing 91.2 µM Z or 31.1 µM BAP with 4.7 µM Kin. The separated shoots rooted and elongated on medium containing 2.5 or 4.9 µM indole-3-butyric acid (IBA). Axillary shoots were induced from in vitro raised plantlets by decapitating them. The axillary shoot-tip explants were used for further multiple shoot buds induction. A maximum of about 150 plantlets were obtained from a single seedling. Hardened and acclimatized plantlets were successfully established in the soil.

Keywords: axillary shoot induction; chillies; decapitation; multiple shoot induction

Subjects: Capsicum chinense; decapitation; in vitro culture, micropropagation; in vitro culture, regeneration; nutrient medium, Murashige and Skoog (MS)

Received: July 12, 2006; Accepted: January 17, 2007; Published: September 1, 2008  Show citation