biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:521-524, 2008 | DOI: 10.1007/s10535-008-0101-9

In vitro clonal propagation of Nyctanthes arbor-tristis

G. R. Rout1,*, A. Mahato1, S. K. Senapati1
1 Plant Biotechnology Division, Regional Plant Resource Centre, Bhubaneswar, Orissa, India

Rapid shoot multiplication of Nyctanthes arbor-tristis L. was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0-1.5 mg dm-3 6-benzylaminopurine (BA), 50 mg dm-3 adenine sulfate (Ads) and 3 % (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg dm-3 BA, 50 mg dm-3 Ads and 0.1 mg dm-3 IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 d on half-strength MS medium supplemented with either indole-3-butyric acid (IBA), IAA or 1-naphthaleneacetic acid (NAA) with 2 % sucrose. Maximum percentage of rooting was obtained on medium having 0.25 mg dm-3 IBA and 0.1 mg dm-3 IAA. About 70 % of the rooted plantlets survived in the greenhouse. The in vitro raised plants were grown normally in the field.

Keywords: growth regulators; medicinal plant; shoot multiplication
Subjects: auxins; in vitro culture, micropropagation; in vitro culture, regeneration; medicinal plants; nutrient medium, Murashige and Skoog (MS); Nyctanthes arbor-tristis

Received: August 15, 2006; Accepted: April 3, 2007; Published: September 1, 2008  Show citation

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Rout, G.R., Mahato, A., & Senapati, S.K. (2008). In vitro clonal propagation of Nyctanthes arbor-tristis. Biologia plantarum, 52(3), 521-524. doi: 10.1007/s10535-008-0101-9
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