biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:525-528, 2008 | DOI: 10.1007/s10535-008-0102-8

Development of a sequence characterized amplified region (SCAR) marker associated with high rooting ability in Larix

H. Li1,2, S. G. Zhang3, J. M. Gao4, C. G. Wang1, Y. Zhang5, L. W. Qi3, L. Chen1, W. Q. Song1,*
1 Laboratory of Cell Biology, College of Life Sciences, Nankai University, Tianjin, P.R. China
2 Department of Horticulture, Tianjin Agricultural University, Tianjin, P.R. China
3 Research Institute of Forestry, Chinese Academy of Forestry, Beijing, P.R. China
4 Department of Agriculture, Tianjin Agricultural University, Tianjin, P.R. China
5 University of Electronic Science and Technology of China, Chendu, P.R. China

In this study, bulked segregant analysis (BSA) was used on Larix leptolepis × Larix olgensis hybrids to identify a random amplified polymorphic DNA (RAPD) marker associated with high rooting ability in larch. Two DNA bulks: H (high rooting ability) bulk and L (low rooting ability) bulk were constructed according to the rooting percentages of the stock plants. Among the 328 primers, only S356 could amplify a specific band, named S356445, which only existed in the H bulk and was further confirmed following selective genotyping of individual hybrids. Grounded on the border sequences, S356445 was converted to a sequence characterized amplified region (SCAR) marker, HRL445, which can be useful in marker-assisted selection (MAS) to screen for larch with high rooting ability. All the results strongly indicated that S356445 and HRL445 were closely associated with high rooting ability in larch.

Keywords: BSA; hybrid; MAS; RAPD; rooting percentages
Subjects: DNA markers; in vitro culture, rooting; Larix leptolepis; Larix olgensis; random amplified polymorphic DNA (RAPD)

Received: August 16, 2006; Accepted: March 1, 2007; Published: September 1, 2008  Show citation

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Li, H., Zhang, S.G., Gao, J.M., Wang, C.G., Zhang, Y., Qi, L.W., Chen, L., & Song, W.Q. (2008). Development of a sequence characterized amplified region (SCAR) marker associated with high rooting ability in Larix. Biologia plantarum, 52(3), 525-528. doi: 10.1007/s10535-008-0102-8
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