biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:538-542, 2008 | DOI: 10.1007/s10535-008-0105-5

An improved protocol for micropropagation of elite genotypes of Simmondsia chinensis (Link) Schneider

A. Singh1, M. P. Reddy1,*, J. S. Patolia1
1 Discipline of Phytosalinity, Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India

An efficient micropropagation protocol was developed for elite male and female genotypes of Simmondsia chinensis using nodal segments. Bud initiation was found to be best on Murashige and Skoog's (MS) medium supplemented with 4.44 µM 6-benzylaminopurine (BAP) and 88.8 µM adenine. Upon sub-culture, 10-15 shoots per explant were obtained when 4.44 µM BAP and 74.0 µM adenine were incorporated in the medium. Increase in KNO3 concentration in the medium improved shoot multiplication rate and in vitro flowering in 20 % of male cultures. Elongated shoots were harvested, pulse treated for 48 h on liquid medium supplemented with 49.0 µM indole-3-butyric acid, 5.40 µM α-naphthaleneacetic acid and 5.71 µM indole-3-acetic acid for root induction and rooting (92 %) was achieved on hormonal free half-strength MS medium supplemented with 1.37 µM chlorogenic acid, 1 % activated charcoal and 2 % sucrose. After successful hardening, plantlets were transferred to greenhouse with 99 % establishment.

Keywords: female; flowering; jojoba; male; multiplication; vitrification
Subjects: auxins; cultivar and genotype differences; flower, development; in vitro culture, micropropagation; nutrient medium, Murashige and Skoog (MS); Simmondsia chinensis

Received: October 24, 2006; Accepted: May 17, 2007; Published: September 1, 2008  Show citation

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Singh, A., Reddy, M.P., & Patolia, J.S. (2008). An improved protocol for micropropagation of elite genotypes of Simmondsia chinensis (Link) Schneider. Biologia plantarum, 52(3), 538-542. doi: 10.1007/s10535-008-0105-5
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    References

    1. Alcaraz, M.L., Ayla-Rocha, B.: Asexual reproduction of Jojoba by layering.-Hort. Sci. 17: 893-96, 1982. Go to original source...
    2. Babic, V., Nescovic, M.: Propagation of three blackberry cultivars from small apical bud in vitro.-J. hort. Sci. 59: 183-185, 1984. Go to original source...
    3. Chaturvedi, H.C., Sharma, M.: In vitro production of cloned plants of Jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long term culture.-Plant Sci. 63: 199-207, 1989. Go to original source...
    4. Collet, G.F.: Improvement to induce rooting of fruit trees in vitro.-Acta Hort. 227: 318-324. 1988. Go to original source...
    5. Driver, J.A., Kuniyuki, A.H.: In vitro propagation of paradox walnut rootstock.-Hort. Sci. 19: 507-509, 1984. Go to original source...
    6. Jacoboni, A., Standarti, A.: Tissue culture of jojoba (Simmondsia chinensis Link).-Acta Hort. 212: 557-560, 1987. Go to original source...
    7. Kaliamoorthy, S., Naidoo, G., Achar, P.: Micropropagation of Harpagophytum procumbens.-Biol. Plant. 52: 191-194, 2008. Go to original source...
    8. Lee, C.W., Palzkill, D.A.: Propagation of jojoba by single node cuttings.-Hort. Sci 19: 841-842, 1984. Go to original source...
    9. Llorente, B.E., Apostolo, N.M.: Effect of different growth regulators and genotype on in vitro propagation of jojoba.-New Zealand J. of Crop and Hort. Sci. 26: 55-62, 1998. Go to original source...
    10. Mandani, A., Lee, C.W., Hogan, L.: In vitro propagation of Simmondsia chinensis via shoot tip culture.-Hort. Sci. 13: 35-37, 1978.
    11. Mills, D., Wenkart, S., Benzioni, A.: Micropropagation of Simmondsia chinensis (jojoba).-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 40. Pp. 380-939. Springer-Verlag, Berlin-Heidelberg 1997. Go to original source...
    12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. plant. 15: 473-497, 1962. Go to original source...
    13. Peare, H.L.: Experiments with growth controlling substances. II. Response of the fruit-tree cuttings to treatment with synthetic root forming substances.-Rep. East Malling Res. Sta. 21: 157-166, 1938.
    14. Rao, M.S., Purohit, S.D.: In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd.-Biol. Plant. 50: 501-506, 2006. Go to original source...
    15. Roussos, P.A., Tolia-Marioli, A., Pontikis, C.A., Kotsias, D.: Rapid multiplication of jojoba seedlings by in vitro culture.-Plant Cell Tissue Organ Cult. 57: 133-137, 1999. Go to original source...
    16. Selvaraj, N., Vasudevan, A., Manikavasagam, M., Ganpathi, A.: In vitro organogenesis and plant formation in cucumber.-Biol. Plant. 50: 123-126, 2006. Go to original source...
    17. Torrey, J.G.: Root hormones and plant growth.-Annu. Rev. Plant Physiol. 27: 435-459, 1976. Go to original source...
    18. Tyagi, R.K., Prakash, S.S.: Genotype-and sex-specific protocol for in vitro micropropagation and medium-term conservation of jojoba.-Biol. Plant. 48: 119-123, 2004. Go to original source...
    19. Yermanos, D.M.: Jojoba-a crop whose time has come.-California Agr. 33: 4-9 and 10-11, 1979.

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