biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:543-547, 2008 | DOI: 10.1007/s10535-008-0106-4

Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data

A. Refoufi1,*, M. A. Esnault1
1 Université de Rennes 1, UMR 6553 Ecobio, Rennes CEDEX, France

Thirty five bands (alleles) from six enzyme systems and fifty seven random amplified polymorphic DNA (RAPD) fragments were selected to analyse the genetic diversity of 33 polyploid wheatgrasses (Triticeae) populations of species Thinopyrum junceiforme and Elytrigia pycnantha, and two hybrids, one pentaploid and one novel 9-ploid. Dice's similarity coefficient, the UPGMA-derived phenograms from RAPD, and allozymes markers showed that the clustering of wheatgrass populations was based on ploidy level. These markers had similar levels of diversity between populations, with high genetic similarity within the same ploidy-level and within population's individuals. The tetraploid Th. junceiforme populations are closely related, with a large similarity distances varied from 0.8 to 1. Based on the isozyme and RAPD analyses, diploid taxa are related to polyploids with similarity coefficients 0.4.

Keywords: Elytrigia pycnantha; isoenzymes; pentaploid hybrid; 9-ploid hybrid; RAPD; Thinopyrum junceiforme
Subjects: amino acids; Elytrigia pycnantha; genetic diversity, biodiversity; ploidy; random amplified polymorphic DNA (RAPD); Thinopyrum junceiforme; wheatgrasses

Received: October 13, 2006; Accepted: June 10, 2007; Published: September 1, 2008  Show citation

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Refoufi, A., & Esnault, M.A. (2008). Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data. Biologia plantarum, 52(3), 543-547. doi: 10.1007/s10535-008-0106-4
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