biologia plantarum

Biologia plantarum 52:548-552, 2008 | DOI: 10.1007/s10535-008-0107-3

Protein profiling in F₁ and F₂ generations of two tomato genotypes differing in ripening time

G. R. Rodríguez^1,*, L. Sequin², G. R. Pratta¹, R. Zorzoli³, L. A. Picardi³

¹ Fac. Cs. Agrarias, UNR, CC 14, CONICET-Cátedra de Genética, Zavalla, Argentina

² E.E. INTA Marcos Juárez, Marcos Juárez, Córdoba, Argentina

³ Fac. Cs. Agrarias, UNR, CC 14, CIUNR-Cátedra de Genética, Zavalla, Argentina

Pericarp polypeptide profiles were analyzed at three ripening stages in the F₁ hybrid and the F₂ population from the cross between the accessions: LA1385 (Lycopersicon esculentum var. cerasiforme) and 804627 (L. esculentum, a homozygous genotype for the nor mutant). Six polymorphic polypeptides were observed in LA1385, while no polymorphic polypeptides among ripening stages was observed in 804627. On the other hand, some polypeptides in the F₁ hybrid were not observed in the parents whereas others were present in both parental genotypes and were unnoticeable in the hybrid genotype. From a cluster analysis on the protein profiles of the F₂ population, the differential expression of proteins allowed to distinguish mature green (MG) stage from the others two stages, while for breaker stage (BR) and red ripe stage, the genetic background was more important in forming groups. The differential expression of proteins could be associated with fruit morphology traits such as a 72 kDa polypeptide present in MG stage with fruit diameter, height and mass and a 47 kDa polypeptide found in BR with fruit shelf life.

Keywords: fruit shelf life; Lycopersicon esculentum var. cerasiforme; nor mutant; SDS-PAGE; tomato fruit ripening

Subjects: fruit ripening; Lycopersicon esculentum; mutagenesis, mutants; proteins; proteins; tomato

Received: December 12, 2006; Accepted: May 25, 2007; Published: September 1, 2008  Show citation