biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:553-556, 2008 | DOI: 10.1007/s10535-008-0109-1

Influences of cefotaxime and carbenicillin on plant regeneration from wheat mature embryos

Y. Yu1,2, Z.-M. Wei1,*
1 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
2 Graduate School of the Chinese Academy of Sciences, Shanghai, China

The influences of cefotaxime and carbenicillin on regeneration potential of wheat (Triticum aestivum L.) mature embryos were investigated. Filter-sterilized cefotaxime enhanced regeneration capacity although it did not affect the average number of shoots per explant. The highest regeneration capacity of 55.4 % was obtained on regeneration medium supplemented with 100 mg dm-3 cefotaxime. Filter-sterilized carbenicillin did not stimulate plant regeneration. However, higher concentration (100 mg dm-3) accelerated callus browning and inhibited the following regeneration. Autoclaved antibiotics at all tested concentrations showed detrimental effects on callus morphogenesis and plant regeneration.

Keywords: Triticum aestivum; antibiotics; embryogenic callus; plant tissue culture
Subjects: in vitro culture, callus; in vitro culture, regeneration; in vitro culture, somatic embryogenesis; nutrient medium, Murashige and Skoog (MS); Triticum aestivum; wheat

Received: January 12, 2007; Accepted: June 4, 2007; Published: September 1, 2008  Show citation

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Yu, Y., & Wei, Z.-M. (2008). Influences of cefotaxime and carbenicillin on plant regeneration from wheat mature embryos. Biologia plantarum, 52(3), 553-556. doi: 10.1007/s10535-008-0109-1
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