biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:616-620, 2008 | DOI: 10.1007/s10535-008-0121-5

High frequency plant regeneration from protoplasts in cotton via somatic embryogenesis

J. Wang1, Y. Sun1, S. Yan1, M. K. Daud1, S. Zhu1,*
1 Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China

A highly reproducible system for efficient plant regeneration from protoplast via somatic embryogenesis was developed in cotton (Gossypium hirsutum L.) cultivar ZDM-3. Embryogenic callus, somatic embryos and suspension culture cells were used as explants. Callus-forming frequency (82.86 %) was obtained in protoplast cultures from suspension culture cells in KM8P medium with 0.45 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.93 µM kinetin (KIN), 1.5 % glucose and 1.5 % maltose. Protocolonies formed in two months with plating efficiency of 14 %. However, the callus-forming efficiencies from other two explants were low. The calli from protoplast culture were transferred to somatic embryo induction medium and 12.7 % of normal plantlets were obtained on medium contained 3 % maltose or 1 % of each sucrose + maltose + glucose, 2.46 µM indole-3-butyric acid (IBA) and 0.93 µM KIN. Over 100 plantlets were obtained from protoplasts derived from three explants. The regenerated plants were transferred to the soil and the highest survival rate (95 %) was observed in transplanting via a new method.

Keywords: ex vitro transplantation; Gossypium hirsutum L; plant growth regulation; sugar combinations
Subjects: auxins; cotton; cytokinins; ex vitro transfer; Gossypium hirsutum; growth analysis, biomass and yield enhancement; in vitro culture, callus; in vitro culture, somatic embryogenesis; protoplasts; sugars

Received: December 14, 2006; Accepted: June 17, 2007; Published: December 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wang, J., Sun, Y., Yan, S., Daud, M.K., & Zhu, S. (2008). High frequency plant regeneration from protoplasts in cotton via somatic embryogenesis. Biologia plantarum, 52(4), 616-620. doi: 10.1007/s10535-008-0121-5
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chen, Z.X., Li, S.J., Yue, J.X., Jiao, G.L., Liu, S.X., She, J.M., Wu, J.Y., Wang, H.B.: Plantlet regeneration from protoplasts isolated from an embryogenic suspension culture of cotton (Gossypium hirsutum L.).-Acta bot. sin. 31: 966-969, 1989.
    2. El-Shihy, O.M., Evans, P.K.: Protoplast isolation, culture and plant regeneration of cotton (Gossypium barbadense L.).-In: Proceedings of 6th International Congress of Plant Tissue and Cell Culture. P. 52. Minnesota 1986.
    3. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968. Go to original source...
    4. Jin, S., Zhang, X., Nie, Y., Guo, X., Liang, S., Zhu, H.: Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton.-Biol. Plant. 50: 519-524, 2006. Go to original source...
    5. Lü, F.B., Zhang, X.L., Liu, J.L.: Protoplast culture and plant regeneration in upland cotton.-Acta agr. boreali-sin. 14: 73-78, 1999.
    6. Mishra, R., Wang, H.Y., Yadav, N.R., Wilkins, T.A.: Development of a highly regenerable elite Acala cotton (Gossypium hirsutum cv. Maxa) - a step towards genotypeindependent regeneration.-Plant Cell Tissue Organ Cult. 73: 21-35, 2003. Go to original source...
    7. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    8. Peeters, M.C., Willems, K., Swennen, R.: Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers.-Plant Cell Rep. 13: 208-211, 1994. Go to original source...
    9. She, J.M., Wu, J.Y., Wang, H.B., Zhou, H.Y., Chen, Z.X., Li, S.J., Yue, J.X.: Somatic embryogenesis and plant regeneration from protoplast culture of cotton (Gossypium hirsutum L.).-Jiangsu J. agr. Sci. 5: 54-60, 1989.
    10. She, J.M., Wu, J.Y., Zhou, H.Y.: High frequency of plating efficiency and plant regeneration from protoplasts of cotton (Gossypium hirsutum L.).-Acta agr. sin. 2: 109-113, 1991.
    11. Srinivasan, C., Vasil, I.K.: Plant regeneration from protoplasts of sugarcane (Saccharum officinarum L.).-Plant Physiol. 126: 41-48, 1986. Go to original source...
    12. Sun, Y.Q., Zhang, X.L., Huang, C., Nie, Y.C., Guo, X.P.: Factors influencing in vitro regeneration from protoplasts of wild cotton (G. klotzschianum A) and RAPD analysis of regenerated plantlets.-Plant Growth Regul. 46: 79-86, 2005a. Go to original source...
    13. Sun, Y.Q., Zhang, X.L., Huang, C., Nie, Y.C., Guo, X.P.: Plant regeneration via somatic embryogenesis from protoplasts of six explants in Coker 201 (Gossypium hirsutum).-Plant Cell Tissue Organ Cult. 82: 309-315, 2005b. Go to original source...
    14. Sun, Y.Q., Zhang, X.L., Jin, S.X., Liang, S.G., Nie, Y.C.: Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum).-Plant Cell Tissue Organ Cult. 75: 247-253, 2003. Go to original source...
    15. Sun, Y.Q., Zhang, X.L., Nie, Y.C., Guo, X.P.: Production of fertile somatic hybrids of Gossypium hirsutum + G. bickii and G. hirsutum + G. stockii via protoplast fusion.-Plant Cell, Tissue Organ Cult. 83: 303-310, 2005c. Go to original source...
    16. Sun, Y.Q., Zhang, X.L., Nie, Y.C., Guo, X.P., Jin, S.X., Liang, S.G.: Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (Gossypium klotzschianum Anderss) via electrofusion.-Theor. appl. Genet. 109: 472-479, 2004. Go to original source...
    17. Trolinder, N.L., Xhixian, C.: Genotype specificity of the somatic embryogenesis response in cotton.-Plant Cell Rep. 8: 133-136, 1989. Go to original source...

    Return to the content