biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:621-626, 2008 | DOI: 10.1007/s10535-008-0122-4

Factors affecting plant regeneration from immature inflorescence of two winter wheat cultivars

M. Kavas1, H. A. Öktem1, M. Yücel1,*
1 Department of Biological Sciences, Middle East Technical University, Ankara, Turkey

Inflorescence explants of two winter wheat cultivars, Triticum durum cv. Kiziltan-91 and T. aestivum cv. Bezostaja-01, were used to evaluate the effects of vernalization period of donor plants, callus age and medium composition on regeneration capacity. Donor plants were grown for 7 d and they were exposed to 4 °C for 1, 2, 3, 4, and 5 weeks. The maximum inflorescence formation was observed as 79 % at 4 weeks and 73 % at 5 weeks of vernalization period for Kiziltan-91 and Bezostaja-01, respectively. Among 6 different callus induction and regeneration mediums, I1-R1 and I3-R3 have to be the best responding mediums for Kiziltan-91 and Bezostaja-01, respectively. In Kiziltan-91, calli induced from donor plants, vernalized for 3 weeks, showed a significantly lower regeneration capacity than counterparts vernalized for 4 and 5 weeks. The highest regeneration capacity of 69 % was obtained from 6-week-old calli produced from 4 weeks vernalized Kiziltan-91 donor plants. In contrast to Kiziltan-91 cultures, the effects of vernalization period and callus age on regeneration capacity were not significant in Bezostaja-01 cultures. The maximum numbers of tillers were obtained from 6-and 15-week-old calli for Bezostaja-01 and Kiziltan-91, respectively. In contrast to vernalization period of donor plants, callus age had no effect on seed number.

Keywords: callus age; plant growth hormones; Triticum aestivum; Triticum durum; vernalization
Subjects: auxins; cultivar and genotype differences; in vitro culture, callus; in vitro culture, regeneration; nutrient medium, Murashige and Skoog (MS); Triticum aestivum; Triticum durum; vernalization; wheat

Received: September 20, 2006; Accepted: June 17, 2007; Published: December 1, 2008  Show citation

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Kavas, M., Öktem, H.A., & Yücel, M. (2008). Factors affecting plant regeneration from immature inflorescence of two winter wheat cultivars. Biologia plantarum, 52(4), 621-626. doi: 10.1007/s10535-008-0122-4
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