biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:627-633, 2008 | DOI: 10.1007/s10535-008-0123-3

Genetic diversity in in vitro-conserved germplasm of Curcuma L. as revealed by RAPD markers

Z. Hussain1, R. K. Tyagi1,*, R. Sharma1, A. Agrawal1
1 Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, India

A set of 30 accessions of five Curcuma species-C. latifolia, C. malabarica, C. manga and C. raktakanta and 13 morphotypes (identified on the basis of morphological markers) of C. longa conserved in the In Vitro Genebank at National Bureau of Plant Genetic Resources, New Delhi, were subjected to RAPD analysis. Of the 200 RAPD primers screened, 21 polymorphic primers were selected for further study. Mean genetic similarities based on Jaccard's similarity coefficient ranged from 0.18 to 0.86 in accessions of cultivated species, i.e., C. longa and from 0.25 to 0.86 in wild species. The dendrogram derived from the RAPD data corroborated the morphological classification of the morphotypes. The efficiency of individual RAPD primers was also compared; primers OPC-20, OPO-06, OPC-01 and OPL-03 were adjudged highly informative in discriminating the germplasm of Curcuma.

Keywords: conservation; in vitro genebank; marker informativeness; morphotypes; turmeric
Subjects: Curcuma species; random amplified polymorphic DNA (RAPD)

Received: September 20, 2006; Accepted: July 29, 2007; Published: December 1, 2008  Show citation

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Hussain, Z., Tyagi, R.K., Sharma, R., & Agrawal, A. (2008). Genetic diversity in in vitro-conserved germplasm of Curcuma L. as revealed by RAPD markers. Biologia plantarum, 52(4), 627-633. doi: 10.1007/s10535-008-0123-3
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