biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:634-641, 2008 | DOI: 10.1007/s10535-008-0124-2

The differences in cell wall composition in leaves and regenerating protoplasts of Beta vulgaris and Nicotiana tabacum

E. Wi¶niewska1, A. Majewska-Sawka1,*
1 Laboratory of Biotechnology, Plant Breeding and Acclimatization Institute, Bydgoszcz, Poland

Cell wall composition in leaves and regenerating leaf-derived protoplasts was studied in Beta vulgaris L. and Nicotiana tabacum L. Several oligosaccharides that characterize arabinogalactan proteins (AGPs) and pectins were visualized in situ by a series of immunocytochemical reactions. The most conspicuous difference between the two species involved the expression of AGP epitopes that bind LM2 and MAC207 in only sugar beet cells; both epitopes being characterized by the presence of glucuronic acid (GlcA). Moreover, B. vulgaris leaves and protoplast-derived cells showed lower content of pectin side-chains bearing (1→4)-β-D-galactose residues as compared to N. tabacum.

Keywords: arabinogalactan proteins; pectins; sugar beet; tobacco
Subjects: Beta vulgaris; in vitro culture, regeneration; Nicotiana tabacum; pectins; proteins; protoplasts; stomata guard cells; sugar beet; tobacco

Received: January 15, 2007; Accepted: August 25, 2007; Published: December 1, 2008  Show citation

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Wi¶niewska, E., & Majewska-Sawka, A. (2008). The differences in cell wall composition in leaves and regenerating protoplasts of Beta vulgaris and Nicotiana tabacum. Biologia plantarum, 52(4), 634-641. doi: 10.1007/s10535-008-0124-2
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