biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:648-653, 2008 | DOI: 10.1007/s10535-008-0126-0

Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis

P. Ray Choudhury1,*, I. P. Singh1, B. George1, A. K. Verma1, N. P. Singh1
1 Biotechnology Unit, Indian Institute of Pulses Research, Kanpur, India

In our present study assessment of genetic diversity and identification of pigeonpea cultivars has been done by employing 76 random amplified polymorphic DNA (RAPD) primers. Out of 796 amplified products, 587 showed polymorphism (73.7 %) and an average of 10.47 bands were amplified per primer. Cluster analysis based on Jaccard's similarity coefficient using UPGMA grouped all the cultivars into three clusters. The cluster I consists of 7 cultivars, cluster II of 11 cultivars in 4 sub-clusters and cluster III 4 cultivars. Two cultivars were not included in any cluster. The clustering was strongly supported by high bootstrap values. Furthermore, high values of the average heterozygosity (Hav) and marker index (MI) also indicated the efficiency of RAPD as a marker system.

Keywords: DNA fingerprinting; molecular marker; DNA amplification; Cajanus cajan
Subjects: Cajanus cajan; cultivar and genotype differences; DNA markers; genetic diversity, biodiversity; pigeopea

Received: February 8, 2007; Accepted: November 15, 2007; Published: December 1, 2008  Show citation

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Ray Choudhury, P., Singh, I.P., George, B., Verma, A.K., & Singh, N.P. (2008). Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis. Biologia plantarum, 52(4), 648-653. doi: 10.1007/s10535-008-0126-0
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    References

    1. Ahmad, F.: Random amplified polymorphic DNA (RAPD) analysis reveals genetic relationships among the annual Cicer species.-Theor. appl. Genet. 98: 657-663, 1999. Go to original source...
    2. Baranger, A., Aubert, G., Arnau, G., Laine, A.L., Deniot, G., Potier, J., Weinachter, C., Lejeune-Henaut, I., Lallemand, J., Burstin, J.: Genetic diversity within Pisum sativum using protein and PCR-based markers.-Theor. appl. Genet. 108: 1309-1321, 2004. Go to original source...
    3. Cao, W., Scoles, G., Hucl, P., Chibbar, R.N.: Phylogenetic relationships of five morphological groups of hexaploid wheat (Triticum aestivum L. em Thell.) based on RAPD analysis.-Genome 43: 724-727, 2000. Go to original source...
    4. Chakrabarti, S.K., Pattanayak, D., Sarkar, D., Chimote, V.P., Naik, P.S.: Stability of RAPD fingerprints in potato: effect of source tissue and primers.-Biol. Plant. 50: 531-536, 2006. Go to original source...
    5. Dangi, R.S., Lagu, M.D., Choudhary, L.B., Ranjekar, P.K., Gupta, V.S.: Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers.-BMC Plant Biol. 4: 13, 2004. Go to original source...
    6. Guillemant, P., Laurence, M.D.: Isolation of plant DNA: a fast, inexpensive, and reliable method.-Plant mol. Biol. Rep. 10: 60-65, 1992. Go to original source...
    7. Huh, M.K., Huh, H.W.: Genetic diversity and population structure of wild tare lentil.-Crop Sci. 41: 1940-1946, 2001. Go to original source...
    8. Karp, A., Edwards, K., Bruford, M., Vosman, B., Morgante, M., Seberg, O., Kremer, A., Boursot, P., Arctander, P., Tautz, D., Hewitt, G.: Newer molecular technologies for biodiversity evaluation: opportunities and challenges.-Nature Biotech. 15: 625-628, 1997. Go to original source...
    9. Kollipara, K.P., Singh, L., Hymowitz, T.: Genetic variation of trypsin and chymotrypsin inhibitors in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives.-Theor. appl. Genet. 88: 986-993, 1994. Go to original source...
    10. Lakhanpaul, S., Chadha, S., Bhat, K.V.: Random amplified polymorphic DNA (RAPD) analysis in Indian mung bean (Vigna radiata (L.) Wilczek) cultivars.-Genetica 109: 227-234, 2000. Go to original source...
    11. Ladizinsky, G., Hamel, H.: Seed protein profile of pigeonpea (Cajanus cajan) and some Atylosia species.-Euphytica 29: 313-317, 1980. Go to original source...
    12. Nei, M.: Estimation of average heterozygosity and genetic distance from a small number of individuals.-Genetics 83: 583-590, 1978. Go to original source...
    13. Panguluri, S.K., Janaiah, K., Govil, J.N., Kumar, P.A., Sharma, P.C.: AFLP fingerprinting in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives.-Genet. Res. Crop Evol. 53: 523-531, 2006. Go to original source...
    14. Pasquet, R.S.: Allozyme diversity of cultivated cowpea, Vigna unguiculata.-Theor. appl. Genet. 101: 211-219, 2002. Go to original source...
    15. Pejic, I., Ajmone-Marson, P., Morgante, M., Kozumplick, V., Castiglioni, P., Taramino, G., Motto, M.: Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs.-Theor. appl. Genet. 97: 1248-1255, 1998. Go to original source...
    16. Powell, W., Morgante, M., Andre, C., Hanafey, M., Voges, J., Tingey, S., Rafalski, A.: A comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis.-Mol. Breed. 2: 225-230, 1996. Go to original source...
    17. Ratnaparkhe, M.B., Gupta, V.S., Ven Murthy, M.R., Ranjekar, P.K.: Genetic finger-printing of pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives using RAPD markers.-Theor. appl. Genet. 91: 893-898, 1995. Go to original source...
    18. Ray Choudhury, P., Kohli, S., Srinivasan, K., Mohapatra, T., Sharma, R.P.: Identification and classification of aromatic rices based on DNA fingerprinting.-Euphytica 118: 243-251, 2001. Go to original source...
    19. Rohlf, F.J.: NTSYS-pc version 1.70 numerical taxonomy and multivariate analysis system.-Applied Biostatistics Inc., Exeter Software, Setauket-New York 1992.
    20. Simioniuc, D., Uptmoor, R., Friedt, W., Ordon, F.: Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs.-Plant Breed. 121: 429-435, 2002. Go to original source...
    21. Taran, B., Zhang, C., WarKentin, T., Tullu, A., Vanderberg, A.: Genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.) based on molecular markers, and morphological and physiological characters.-Genome 48: 257-272, 2005. Go to original source...
    22. Weder, J.K.P.: Identification of plant food raw material by RAPD-PCR: Legumes.-J. Agr. Food Chem. 50: 4456-4463, 2002. Go to original source...
    23. Yap, I.V., Nelson, R.: WinBoot: A program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms.-IRRI Discussion Paper Series No. 14, IRRI, Los Baños 1995.

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