biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:654-659, 2008 | DOI: 10.1007/s10535-008-0127-z

Detection of DNA ladder during cotyledon senescence in cotton

Q. E. Xie1, I. D. Liu1, S. X. Yu2, R. F. Wang1, Z. X. Fan1, Y. G. Wang1, F. F. Shen1,*
1 State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, P.R. China
2 Cotton Research Institute, the Chinese Agricultural Academy of Sciences, Anyang, Henan, P.R. China

The asynchronous programmed cell death (PCD) in different regions of the cotton cotyledon was studied during senescence. We showed that changes in chlorophyll contents do not occur at the same time in different parts of the cotyledon. By using light microscopy (LM) and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labelling (TUNEL) methods, the symptoms of cell death were detected in cotyledon margins earlier than in the central part. DNA ladders were detected by gel electrophoresis in senescent cotyledon margins and in the centre respectively, but not in the whole cotyledons.

Keywords: chlorophyll content; Gossypium hirsutum; programmed cell death; DNA fragmentation
Subjects: chlorophyll a,b; cotton; cotyledon, cotyledonary node; DNA markers; Gossypium hirsutum; in vitro culture, cell suspension

Received: September 20, 2006; Accepted: August 8, 2007; Published: December 1, 2008  Show citation

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Xie, Q.E., Liu, I.D., Yu, S.X., Wang, R.F., Fan, Z.X., Wang, Y.G., & Shen, F.F. (2008). Detection of DNA ladder during cotyledon senescence in cotton. Biologia plantarum, 52(4), 654-659. doi: 10.1007/s10535-008-0127-z
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