biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:703-708, 2008 | DOI: 10.1007/s10535-008-0135-z

Differential expression of wheat transcriptomes in response to varying cadmium concentrations

O. Cebeci1, B. Kokturk1, N. Ergen1, L. Ozturk1, I. Cakmak1, H. Budak1,*
1 Faculty of Engineering and Natural Sciences, Biological Sciences and Bio Engineering Program, Sabanci University, Istanbul, Turkey

This study aims to understand the changes in the transcriptome of durum wheat (Tricitum durum cv. Balcali-85) upon exposure to varying Cd concentrations using mRNA differential display (mRNA DD) technique. Sequence analyses of the two heavily induced genes upon exposure to Cd showed high homology to NADH dehydrogenase subunit 1 (EC907725) and PsaC gene encoding a photosystem 1 (PS 1) 9 kDa subunit protein (EC907731). Additionally, three differentially expressed genes (EC907726, EC907729 and EC907730) were identified. Their sequence analyses revealed no significant homologies to known genes. The expressions of NADH dehydrogenase subunit 1 and PsaC genes were confirmed by Northern blot analysis and quantified by real time PCR. This is the first report for the induction of NADH dehydrogenase subunit 1 gene during Cd stress in wheat.

Keywords: cadmium-responsive genes; functional genomics; mRNA differential display; Triticum durum
Subjects: cDNA cloning; heavy metals; photosystem 1,2; polymerase chain reaction (PCR); Triticum durum; wheat

Received: February 28, 2007; Accepted: August 7, 2007; Published: December 1, 2008  Show citation

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Cebeci, O., Kokturk, B., Ergen, N., Ozturk, L., Cakmak, I., & Budak, H. (2008). Differential expression of wheat transcriptomes in response to varying cadmium concentrations. Biologia plantarum, 52(4), 703-708. doi: 10.1007/s10535-008-0135-z
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