biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:709-712, 2008 | DOI: 10.1007/s10535-008-0136-y

In vitro cormlet development in Crocus sativus

K. D. Sharma1,*, R. Rathour1, R. Sharma1, S. Goel1, T. R. Sharma2, B. M. Singh1
1 Advanced Centre of Hill Bioresources and Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India
2 National Research Centre on Plant Biotechnology, IARI, New Delhi, India

An improved protocol for generation of viable cormlets from tissue culture derived shoots of saffron has been developed. Multiple shoots were generated from apical buds, small corms and in vitro developed single shoots. Bunches of two to three shoots when cultured on half strength Murashige and Skoog (MS) medium containing 3 mg dm-3 benzyladenine (BA) and 80 g dm-3 sucrose developed 1.89 cormlets per shoot bunch with an average fresh mass of 1.18 g. It took nine months from culture of apical buds to the harvest of cormlets but under field conditions 22 months. Sucrose appeared to be essential for cormlet induction as no cormlets were developed in the medium devoid of sucrose and only 0.29 per shoot in medium containing mannitol. In vitro derived cormlets sprouted from apical and axillary buds on MS medium containing 12 mg dm-3 BA, 3 mg dm-3 indolebutyric acid and 30 g dm-3 sucrose. Daughter cormlet formation from in vitro derived cormlets was also observed.

Keywords: apical buds; growth regulators; micropropagation; multiple shoots; saffron; sprouting; sucrose
Subjects: auxins; Crocus sativus; cytokinins; in vitro culture, micropropagation; in vitro culture, regeneration; nutrient medium, Murashige and Skoog (MS); saffron; sugars

Received: September 18, 2006; Accepted: June 4, 2007; Published: December 1, 2008  Show citation

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Sharma, K.D., Rathour, R., Sharma, R., Goel, S., Sharma, T.R., & Singh, B.M. (2008). In vitro cormlet development in Crocus sativus. Biologia plantarum, 52(4), 709-712. doi: 10.1007/s10535-008-0136-y
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