biologia plantarum

Biologia plantarum 52:723-726, 2008 | DOI: 10.1007/s10535-008-0139-8

Induction of in vitro flowering in the orchid Dendrobium Sonia 17

C. S. Tee^1,*, M. Maziah¹, C. S. Tan²

¹ Faculty of Biotechnology and Biomolecular Sciences, Univeristi Putra Malaysia, Serdang, Malaysia

² Malaysian Agricultural Research and Development Institute, Serdang, Malaysia

In this study, Dendrobium Sonia 17 plantlets were used to induce in vitro flowering. Inflorescences were induced and rooting was inhibited in the half-strength Murashige and Skoog medium containing 20 µM N ⁶-benzyladenine (BA). The medium with high P and low N contents was effective to induce inflorescences while the medium with low P and high N contents was only effective to promote forming of shoots. In addition, the induced in vitro inflorescences were able to multiply and maintain without exhibiting a distinctive vegetative phase. Different morphologies of in vitro flowers such as incomplete flower structures, abnormal and unresupinated in vitro flowers were observed.

Keywords: cytokinin; phytohormone; rooting

Subjects: cytokinins; Dendrobium; flower, development; in vitro culture, flowering; in vitro culture, regeneration; in vitro culture, rooting; nutrient medium, Murashige and Skoog (MS)

Received: November 15, 2006; Accepted: July 20, 2007; Published: December 1, 2008  Show citation