biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:735-739, 2008 | DOI: 10.1007/s10535-008-0142-0

Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium

M. Chandrika1, Thoyajaksha1, V. Ravishankar Rai1,*, K. Ramachandra Kini1
1 Department of Studies in Applied Botany and Biotechnology, University of Mysore, Mysore, India

In the present study, a polymerase chain reaction (PCR)-based method namely inter simple sequence repeat (ISSR) was employed to assess genetic stability in tissue culture-derived Dictyospermum ovalifolium plantlets. To study genomic stability of micropropagated plants, 14 individuals were randomly tagged among a population of 2500 regenerants and were compared with single donor mother plant. A total of 51 clear and reproducible bands ranging from 200 bp to 2.1 kb were scored corresponding to an average of 3.64 bands per primer. Two of the 51 bands were polymorphic (3.92 %) among 14 individuals, thus indicating the occurrence of low level genomic variation in the micropropagated plants. Cluster analysis indicates that genetic similarity values were 0.978 which allows classification of the plants to distinct groups. Further an attempt was made to reintroduce the micropropagated plants into their natural habitat. Over one thousand six hundred fifty plants were successfully established.

Keywords: ISSR molecular marker; PCR; micropropagation; reintroduction; conservation
Subjects: Dictyospermum ovalifolium; genetic stability; in vitro culture, growth control; in vitro culture, micropropagation; in vitro culture, regeneration; polymerase chain reaction (PCR)

Received: December 6, 2006; Accepted: June 5, 2007; Published: December 1, 2008  Show citation

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Chandrika, M., Thoyajaksha,, Ravishankar Rai, V., & Ramachandra Kini, K. (2008). Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium. Biologia plantarum, 52(4), 735-739. doi: 10.1007/s10535-008-0142-0
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