biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:749-753, 2008 | DOI: 10.1007/s10535-008-0145-x

Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains

K. V. Sumesh1, P. Sharma-Natu1, M. C. Ghildiyal1,*
1 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India

Wheat (Triticum aestivum L.) cvs. HD 2285 (relatively tolerant) and WH 542 (susceptible) were exposed to ambient and elevated temperature (3-4 °C higher) in open top chambers during post anthesis period. The grain yield components were determined at the time of maturity. In order to elucidate the basis of differential tolerance of these cultivars, the excised developing grains (20 d after anthesis) of ambient grown plants were incubated at 15, 25, 35 and 45 °C for 2 h and then analysed for the activities of soluble starch synthase (SSS), granule bound starch synthase (GBSS), kinetic parameters of SSS and content of heat shock protein (HSP 100). The elevated temperature during grain development significantly decreased grain growth in WH 542 whereas no such decrease was observed in HD 2285. High temperature tolerance of HD 2285 was found to be associated with higher catalytic efficiency (Vmax/Km) of SSS at elevated temperature and higher content of HSP 100.

Keywords: grain growth; granule bound starch synthase; soluble starch synthase; Triticum aestivum
Subjects: cultivar and genotype differences; heat stress, high temperature; in vitro culture, growth control; temperature, high; Triticum aestivum; wheat

Received: February 16, 2007; Accepted: November 17, 2007; Published: December 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sumesh, K.V., Sharma-Natu, P., & Ghildiyal, M.C. (2008). Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains. Biologia plantarum, 52(4), 749-753. doi: 10.1007/s10535-008-0145-x
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Asana, R.D., Williams, R.F.: The effect of temperature stress on grain development in wheat.-Aust. J. agr. Res. 16: 1-13, 1965. Go to original source...
    2. Boston, R.S., Viitanen, P.V., Vierling, E.: Molecular chaperones and protein folding in plants.-Plant mol. Biol. 32: 191-222, 1996. Go to original source...
    3. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    4. Chaudhary-Mehra, R., Shukla, D.S., Pande, P.C.: Biochemical basis of high temperature tolerance in developing grains of wheat (Triticum aestivum L.).-Indian J. exp. Biol. 32: 296-298, 1994.
    5. Chinoy, J.J.: Correlation between yield of wheat and temperature during ripening of grain.-Nature 159: 442-444, 1947. Go to original source...
    6. Engvall, E., Perlmann, P.J.: Enzyme linked immunosorbent assay (ELISA) III. Quantification of specific antibodies by enzyme labelled anti-immunoglobulin in antigen-coated tubes.-J. Immunol. 109: 129-135, 1972. Go to original source...
    7. Fersht, A.: Enzyme Structure and Mechanism.-Freeman, New York 1985.
    8. Fischer, R.A., Maurer, R. Drought resistance in spring wheat cultivars I. Grain yield response.-Aust. J. agr. Res. 29: 897-907, 1978. Go to original source...
    9. George, W.S., Roshie, B., Keeling, P.L.: Heat stress during grain filling in maize: effects on carbohydrate storage and metabolism.-Aust. J. Plant Physiol. 21: 829-841, 1994. Go to original source...
    10. Ghildiyal, M.C., Sirohi, G.S.: Photosynthesis and sink efficiency of different species of wheat.-Photosynthetica 20: 102-106, 1986.
    11. Howard, A.: Crop Production in India.-Oxford University Press, London 1924.
    12. Iba, K.: Acclimation response to temperature stress in higher plants: approaches of gene engineering for temperature tolerance.-Annu. Rev. Plant Biol. 53: 225-245, 2002. Go to original source...
    13. Jenner, C.F.: Starch synthesis in the kernel of wheat under high temperature conditions.-Aust. J. Plant Physiol. 21: 791-806, 1994. Go to original source...
    14. Jenner, C.F., Denyer, K., Guerin, J.: Thermal characteristics of soluble starch synthase from wheat endosperm.-Aust. J. Plant Physiol. 22: 703-709, 1995. Go to original source...
    15. Jenner, C.F., Siwek, K., Hawker, J.S.: The synthesis of (14C) starch from (14C) sucrose in isolated wheat grains is dependent upon the activity of soluble starch synthase.-Aust. J. Plant Physiol. 20: 329-335, 1993. Go to original source...
    16. Katiyar-Agarwal, S., Agarwal, M., Grover, A.: Heat-tolerant basmati rice engineered by over-expression of HSP 101.-Plant mol. Biol. 51: 677-686, 2003. Go to original source...
    17. Keeling, P.L., Bacon, P.J., Holt, D.C.: Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase.-Planta 191: 342-348, 1993. Go to original source...
    18. Keeling, P.L., Banisadr, R., Barone, L., Vasserman, B.P., Singletary, G.W.: Effect of temperature on enzymes in the pathway of starch biosynthesis in developing wheat and maize grain.-Aust. J. Plant Physiol. 21: 807-827, 1994. Go to original source...
    19. Khyse-Anderson, J.: Electroblotting of multiple gels: a simple apparatus without buffer for rapid transfer of proteins from polyacrylamide to nitrocellulose.-J. biochem. biophys. Methods 10: 203-209, 1984. Go to original source...
    20. Leadley, P.W., Drake, B.G.: Open top chambers for exposing plant canopies to elevated CO2 concentration and for measuring net gas exchange.-Vegetatio 104/105: 3-15, 1993. Go to original source...
    21. Lee, Y.R.J., Nagao, R.T., Key, J.L.: A soybean 100 kDa heat shock protein complements a yeast HSP 104 deletion mutant in acquiring thermotolerance.-Plant Cell 6: 1889-1897, 1994. Go to original source...
    22. Leloir, L.E., Goldenberg, S.H.: Synthesis of glycogen from uridine diphosphate glucose in liver.-J. biol. Chem. 235: 919-923, 1960. Go to original source...
    23. Maestri, E., Klueva, N., Perrotta, C., Gulli, M., Nguyen, H.T., Marmiroli, N.: Molecular genetics of heat tolerance and heat shock proteins in cereals.-Plant mol. Biol. 48: 667-681, 2002. Go to original source...
    24. McDonald, G.K., Sutton, B.G., Ellison, F.W.: The effect of time of sowing on the grain yield of irrigated wheat in the Naomi Valley, New South Wales.-Aust. J. agr. Res. 34: 229-240, 1983. Go to original source...
    25. Morell, M.K., Rahman, S., Regina, A., Appels, R., Li, Z.: Wheat starch biosynthesis.-Euphytica 119: 55-58, 2001. Go to original source...
    26. Pareek, A., Singla, S.L., Grover, A.: Immunological evidence for accumulation of two high molecular weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera.-Plant mol. Biol. 29: 293-301, 1995. Go to original source...
    27. Prakash, P., Sharma-Natu, P., Ghildiyal, M.C.: High temperature effect on starch synthase activity in relation to grain growth in wheat cultivars.-Indian J. Plant Physiol. (Special Issue) 8: 390-398, 2003.
    28. Prakash, P., Sharma-Natu, P., Ghildiyal, M.C.: Effect of different temperature on starch synthase activity in excised grains of wheat cultivars.-Indian J. exp. Biol. 42: 227-230, 2004.
    29. Rijven, A.H.G.C.: Heat inactivation of starch synthase in wheat endosperm tissue.-Plant Physiol. 81: 448-453, 1986. Go to original source...
    30. Sanchez, Y., Lindquist, S.L.: HSP 104 required for induced thermotolerance.-Science 248: 1112-1115, 1990. Go to original source...
    31. Schirmer, E.C., Lindquist, S., Vierling, E.: An Arabidopsis heat shock protein compliments a thermotolerance defect in yeast.-Plant Cell 6: 1899-1909, 1994. Go to original source...
    32. Shewmaker, C.K., Stalker, D.M.: Modifying starch biosynthesis with transgenes in potatoes.-Plant Physiol 100: 1083-1086, 1992. Go to original source...
    33. Shi, Y.C., Seib, P.A., Bernardin, J.E.: Effects of temperature during grain filling on starches from six wheat cultivars.-Cereal Chem. 71: 369-383, 1994.
    34. Singla, S.L., Pareek, A., Grower, A.: Distribution patterns of 104 kDa stress associated protein in rice reveal its constitutive accumulation in seeds and disappearance from the just-emerged seedlings.-Plant mol. Biol. 37: 911-919, 1998. Go to original source...
    35. Tester, R.F., Morrison, W.R., Ellis, R.H., Piggott, J.R., Batts, G.R., Wheeler, T.R., Morison, J.I.L., Hadley, P., Ledward, D.A.: Effects of elevated growth temperature and carbon dioxide levels on some physiochemical properties of wheat starch.-J. Cereal Sci. 22: 63-71, 1995. Go to original source...
    36. Vierling, E.: The roles of heat shock proteins in plants.-Annu. Rev. Plant. Physiol. Plant mol. Biol. 42: 579-620, 1991. Go to original source...
    37. Wahid, A., Close T.J.: Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves.-Biol. Plant. 51: 104-109, 2007. Go to original source...
    38. Wang, S., Liu, L., Chen, C., Chen L.: Regulation of granule-bound starch synthase I gene expression in rice leaves by temperature and drought stress.-Biol. Plant. 50: 537-541, 2006. Go to original source...
    39. Wardlaw, I.F., Dawson, I.A., Munibi, P.: The tolerance of wheat to high temperatures during reproductive growth II. Grain development.-Aust. J. agr. Res. 40: 15-24, 1989. Go to original source...
    40. Wiegand, C.L., Cuellar, J.A.: Duration of grain filling and kernel weight of wheat as affected by temperature.-Crop Sci. 21: 95-101, 1981. Go to original source...
    41. Zahedi, M., Sharma, R., Jenner, C.F.: Effects of high temperature on grain growth and on the metabolites and enzymes in the starch-synthesis pathway in the grains of two wheat cultivars differing in their responses to temperature.-Funct. Plant Biol. 30: 291-300, 2003. Go to original source...

    Return to the content