biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:767-770, 2008 | DOI: 10.1007/s10535-008-0149-6

Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea

P. Sharma1, R. Bhardwaj1,*, N. Arora1, H. K. Arora1, A. Kumar2
1 Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, India
2 Serum Institute of India Ltd., Pune, India

The effects of 28-homobrassinolide (HBL) on nickel uptake, protein content and activities of antioxidative enzymes were determined in the seedlings of Brassica juncea L. The seeds were treated with different concentrations (0, 0.01, 1 and 100 nM) of HBL for 8 h and then sown in the Petri plates containing various concentrations (0, 25, 50 and 100 mg dm-3) of nickel. After 7 d, observations were made on shoot and root length, Ni uptake, protein content and activities of antioxidative enzymes (guaiacol peroxidase, catalase, glutathione reductase, ascorbate peroxidase and superoxide dismutase). The growth of seedlings was inhibited by Ni, however, less after HBL pre-treatment. The protein content and antioxidative enzyme activities were also increased by HBL treatment.

Keywords: antioxidative enzymes; brassinosteroids; heavy metal toxicity; Indian mustard
Subjects: antioxidants, antioxidant enzymes; ascorbate peroxidase; Brassica juncea; catalase; glutathione reductase; heavy metals; in vitro culture, growth control; in vitro culture, regeneration; in vitro culture, rooting; proteins; superoxide dismutase (SOD)

Received: August 4, 2007; Accepted: June 8, 2008; Published: December 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sharma, P., Bhardwaj, R., Arora, N., Arora, H.K., & Kumar, A. (2008). Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea. Biologia plantarum, 52(4), 767-770. doi: 10.1007/s10535-008-0149-6
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aebi, H.: Catalase.-In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 673-684. Verlag-Chemie, Weinham 1974. Go to original source...
    2. Ali, B., Hayat, S., Ahmad, A.: Response of germinating seeds of Cicer arietinum to 28-homobrassinolide and/or potassium.-J. gen. appl. Plant Physiol. 31: 55-63, 2005.
    3. Allen, S.E., Grimshaw, H.M., Parkinson, J.A., Quarmby, C., Roberts, J.D.: Chemical Analysis.-In: Chapmann, S.B. (ed.): Methods in Plant Ecology. Pp. 424-426. Blackwell Scientific Publications, Oxford-London 1976.
    4. Bailey, N.T.J.: Statistical Methods in Biology (3rd Ed).-The English University Press, London 1995.
    5. Bajguz, A.: Blockage of heavy metal accumulation in Chlorella vulgaris cells by 24-epibrassinolide.-Plant Physiol. Biochem. 38: 797-801, 2000a. Go to original source...
    6. Bajguz, A.: Effects of brassinosteroids on nucleic acids and protein in cultured cells in Chlorella vulgaris.-Plant Physiol. Biochem. 38: 209-215, 2000b. Go to original source...
    7. Bhardwaj, R., Arora, H.K., Nagar, P.K., Thukral, A.K.: Brassinosteroids - a novel group of plant hormones.-In: Trivedi, P.C. (ed.): Plant Molecular Physiology - Current Scenario and Future Projections. Pp. 58-84. Aaviskar Publisher, Jaipur 2006.
    8. Bilkisu, A.A., Xiao-Gang, G., Qing-Lei, G., Yong-Hua, Y.: Brassinolide amelioration of aluminium toxicity in mungbean seedling growth.-J. Plant Nutr. 26: 1725-1734, 2003. Go to original source...
    9. Cao, X., Ma, L.Q., Tu, C.: Antioxidative responses to arsenic in the arsenic-hyperaccumulator Chinese brake fern (Pteris vittata L.).-Environ. Pollut. 128: 317-325, 2004. Go to original source...
    10. Carlberg, I., Mannervik, B.: Purification of the flavoenzyme glutathione reductase from rat liver.-J. biol. Chem. 250: 5475-5480, 1975. Go to original source...
    11. Clouse, S.D., Sasse, J.M.: Brassinosteroids: essential regulators of plant growth and development.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 427-451, 1998. Go to original source...
    12. Dhaubhadel, S., Browning, K.S., Gallie, D.R., Krishna, P.: Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress.-Plant J. 29: 681-691, 2002. Go to original source...
    13. Dhaubhadel, S., Chaudhary, S., Dobinson, K.F., Krishna, P.: Treatment with 24-epibrassinolide (a brassinosteroid) increases the basic thermotolerance of Brassica napus tomato seedlings.-Plant mol. Biol. 40: 333-342, 1999. Go to original source...
    14. El-Shintinawy, F., El-Ansari, A.: Differential effect of Cd2+ and Ni2+ on amino acid metabolism in soybean seedling.-Biol. Plant. 43: 74-84, 2000. Go to original source...
    15. Fariduddin, Q., Ahmad, A., Hayat, S.: Response of Vigna radiata to foliar application of 28-homobrassinolide and kinetin.-Biol. Plant. 48: 465-468, 2004. Go to original source...
    16. Hayat, S., Ahmad, A.: Soaking seeds of Lens culinaris with 28-homobrassinolide increased nitrate reductase activity and grain yield in the field in India.-Ann. appl. Biol. 143: 121-124, 2003. Go to original source...
    17. Janeczko, A., Koscielniak, J., Pilipowicz, M., Szarek-Lukaszewsa, G., Skoczowspi, A.: Protection of winter rape photosystem 2 by 24-epibrassinolide under cadmium stress.-Photosynthetica 43: 293-298, 2005. Go to original source...
    18. Janeczko, A. Gullner, G., Skoczowski, A., Dubert, F., Barna, B.: Effects of brassinosteroid infiltration prior to cold treatment on ion leakage and pigment contents in rape leaves.-Biol. Plant. 51: 355-358, 2007. Go to original source...
    19. Kalinich, J.F., Mandava, N.B., Todhunter, J.A.: Relationship of nucleic acid metabolism on brassinolide-induced responses in beans.-Plant Physiol. 120: 207-214, 1985. Go to original source...
    20. Kaur, S., Bhardwaj, R.: Brassinosteroids regulated heavy metals uptake in Brassica campestris L.-In: Annual Meeting of the American Society of Plant Biologists "Plant Biology 2003". P. 628. Honolulu 2003.
    21. Kono, Y.: Generation of superoxide radical during autooxidation of hydroxylamine and an assay for superoxide dismutase.-Arch. Biochem. Biophys. 186: 189-195, 1978. Go to original source...
    22. Kovtun, Y., Chiu, W.L., Tena Sheen, J.: Functional analysis of oxidative stress - activated mitogen - activated protein kinase cascade in plants.-Proc. nat. Acad. Sci. USA 97: 2940-2945, 2000. Go to original source...
    23. Lin, Y.C., Kao, C.H.: Proline accumulation induced by excess nickel in detached rice leaves.-Biol. Plant. 51: 351-354, 2007. Go to original source...
    24. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.L.: Protein measurement with Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    25. Maksimoviĉ, I., Kastori, R., Krstiĉ, L., Lukoviĉ, J.: Steady presence of cadmium and nickel affects root anatomy, accumulation and distribution of essential ions in maize seedlings.-Biol. Plant. 51: 589-592, 2007. Go to original source...
    26. Mandava, N.B.: Plant growth promoting brassinosteroids.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 23-52, 1988. Go to original source...
    27. Mazorra, L.M., Nunez, M., Hechavarria, M., Coll, F., Sanchez-Blanco, M.J.: Influence of brassinosteroids on antioxidants enzymes activity in tomato under different temperature.-Biol. Plant. 45: 593-596, 2002. Go to original source...
    28. Meharg, A.A.: The role of the plasmalemma in metal tolerance in angiosperms.-Physiol. Plant. 88: 191-198, 1993. Go to original source...
    29. Metwally, A., Finkemeier, I., Georgi, M., Dietz, K.J.: Salicylic acid alleviates the cadmium toxicity in barley seedlings.-Plant Physiol. 132: 272-281, 2003. Go to original source...
    30. Nakano, Y., Asada, A.: Hydrogen peroxide is scavenged by ascorbate specific-peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
    31. Nunez, M., Mazzafera, P., Mazorra, L.M., Siqueira, W.J., Zullo, M.A.T.: Influence of brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl.-Biol. Plant. 47: 67-70, 2003. Go to original source...
    32. Ozdemir, F., Bor, M., Demiral, T., Turkan, I.: Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress.-Plant Growth Regul. 42: 203-211, 2004. Go to original source...
    33. Panda, S.K., Chaudhury, I., Khan, M.H.: Heavy metals induced lipid peroxidation and affect antioxidants in wheat leaves.-Biol. Plant. 46: 289-294, 2003. Go to original source...
    34. Putter, J.: Peroxidase.-In: Bergmeyer, H.U. (ed): Methods of Enzymatic Analysis. Pp. 685-690. Verlag-Chemie, Weinham 1974. Go to original source...
    35. Sharma, P., Bhardwaj, R.: Effect of 24-epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress.-Acta Physiol. Plant. 29: 259-263, 2007a. Go to original source...
    36. Sharma, P., Bhardwaj, R.: Effect of 24-epibrassinolide on seed germination, seedling growth and heavy metals uptake in Brassica juncea L.-J. gen. appl. Plant. Physiol. 33: 59-73, 2007b.
    37. Vahala, J., Keinanen, M., Schutzendubel, A., Polle, A., Kangasjarvi, J.: Differential effects of elevated ozone on two hybrid aspen genotypes predisposed to chronic ozone fumigation role of ethylene and salicylic acid.-Plant Physiol. 132: 196-205, 2003. Go to original source...

    Return to the content