biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:775-778, 2008 | DOI: 10.1007/s10535-008-0151-z

Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue

D. Skálová1, M. Dziechciarková1, A. Lebeda1,*, E. Křístková1, B. Navrátilová1
1 Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc-Holice, Czech Republic

Embryo-rescue was used to facilitate interspecific hybridization of Cucumis anguria L. and C. zeyheri Sond. Embryos were excised from developing fruits at one week intervals for six weeks after hand pollination. Medium containing coconut water was the most suitable for initial germination, and a medium with ascorbic acid was the best for embryo development and plant recovery. Viable plants were obtained from embryos and these plants showed morphological characteristics different from both parents. The analysis of the leucine aminopeptidase (LAP) locus revealed three hybrid types, H1.1, H1.2 and H2.

Keywords: crossability; isozyme analyses; leucine aminopeptidase; phosphoglucomutase
Subjects: ascorbic acid; auxins; Cucumis anguria; Cucumis zeyheri; cytokinins; gibberellic acid, gibberellins; in vitro culture, somatic embryogenesis; nutrient medium, Murashige and Skoog (MS)

Received: September 24, 2007; Accepted: May 5, 2008; Published: December 1, 2008  Show citation

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Skálová, D., Dziechciarková, M., Lebeda, A., Křístková, E., & Navrátilová, B. (2008). Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue. Biologia plantarum, 52(4), 775-778. doi: 10.1007/s10535-008-0151-z
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