biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:5-10, 2009 | DOI: 10.1007/s10535-009-0002-6

In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum

A. A. Meratan1, S. M. Ghaffari1, V. Niknam2,*
1 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I.R. Iran
2 Department of Plant Sciences, School of Biology, University of Tehran, Tehran, I.R. Iran

The effect of various hormonal combinations on callus formation and regeneration of shoot and root from leaf derived callus of Acanthophyllum sordidum Bunge ex Boiss. has been studied. Proteins and activity of antioxidant enzymes were also evaluated during shoot and root organogenesis from callus. Calli were induced from leaf explants excised from 30-d-old seedlings grown on Murashige and Skoog medium containing 4.52 µM 2,4-dichlorophenoxyacetic acid + 4.65 µM kinetin. Maximum growth of calli and the most efficient regeneration of shoots and roots occurred with 2.69 µM 1-naphthalene acetic acid (NAA), 2.69 µM NAA + 4.54 µM thidiazuron and 2.46 µM indole-3-butyric acid. Protein content decreased in calli and increased significantly during regeneration of shoots from callus. Superoxide dismutase activity decreased in calli comparing to that of seedlings, then increased in regenerated shoots and roots. High catalase activity was detected in seedlings and regenerated shoots, whereas high peroxidase activity was observed in calli and regenerated roots.

Keywords: callus; Caryophyllaceae; catalase; peroxidase; protein; regeneration; superoxide dismutase; tissue culture
Subjects: Acanthophyllum sordidum; auxins; catalase; cytokinins; in vitro culture, callus; in vitro culture, indirect regeneration; in vitro culture, rooting; peroxidase; proteins; superoxide dismutase (SOD)

Received: December 6, 2006; Accepted: November 29, 2007; Published: March 1, 2009  Show citation

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Meratan, A.A., Ghaffari, S.M., & Niknam, V. (2009). In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum. Biologia plantarum, 53(1), 5-10. doi: 10.1007/s10535-009-0002-6
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