biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:125-128, 2009 | DOI: 10.1007/s10535-009-0017-z

RAPD markers associated with quercetin accumulation in Psidium guajava

I. A. Feria-Romero1,*, H. Astudillo-de la Vega2, M.A. Chavez-Soto2, E. Rivera-Arce2, M. López3, H. Serrano3, X. Lozoya2
1 Doctorado en Ciencias Biológicas, UAM, México, D.F., México
2 Centro Medico Nacional Siglo XXI, IMSS, México, D.F., México
3 Universidad Autonoma Metropolitana, México, D.F., México

We used a random amplified polymorphic DNA (RAPD) amplification method to identify molecular markers associated with high quercetin accumulation in the leaves of Psidium guajava L. trees, selected from four different Mexican agronomic regions. We identified six polymorphic RAPD fragments of 620, 590, 370, 690, 480 and 460 bp among individuals of P. guajava. Genetic linkage disequilibrium analysis revealed that three RAPD profiles considered as DNA markers (620/590 bp, 370 bp and 480/460 bp) had a positive, direct association with quercetin content. These informative molecular markers can be used for selective identification of plants with the highest accumulation of flavonoids.

Keywords: guava leaves; DNA profile; linkage disequilibrium; flavonoid accumulation; QTL
Subjects: flavonoids; guava; polymerase chain reaction (PCR); Psidium quajava; quantitative trait loci (QTL); quercitin; random amplified polymorphic DNA

Received: February 6, 2007; Accepted: August 28, 2007; Published: March 1, 2009  Show citation

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Feria-Romero, I.A., Astudillo-De la Vega, H., Chavez-Soto, M.A., Rivera-Arce, E., López, M., Serrano, H., & Lozoya, X. (2009). RAPD markers associated with quercetin accumulation in Psidium guajava. Biologia plantarum, 53(1), 125-128. doi: 10.1007/s10535-009-0017-z
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    References

    1. Becerra, V., Paredes, M.: [Use of biochemical and molecular markers in genetic diversity studies.]-Agr. tec. 60: 270-281, 2000. [In Spanish]
    2. Excoffier, L., Slatkin, M.: Maximum likelihood estimation of molecular haplotype frequencies in a diploid population.-Mol. Biol. Evol. 12: 921-927, 1995.
    3. Havsteen, B.H.: The biochemistry and medical significance of the flavonoids.-Pharmacol. therap. 96: 67-202, 2002. Go to original source...
    4. Lewontin, R.: The detection of linkage disequilibrium in molecular sequence data.-Genetics 140: 377-388, 1995. Go to original source...
    5. Lewontin, R.: The interaction of selection and linkage II. Optimum models.-Genetics 30: 757-782, 1964. Go to original source...
    6. Lozoya, X., Meckes, M., Abou-Zaid, M., Tortoriello, J., Nozzolillo, C., Thor, J.: Quercetin glycosides in Psidium guajava L. leaves and determination of a spasmolytic principle.-Arch. med. Res. 25: 11-15, 1994.
    7. Lozoya, X., Reyes-Morales, H., Chavez-Soto, M.A., Martinez-Garcia, M.C., Soto-Gonzalez, Y., Doubova, S.V.: Intestinal anti-spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease.-J. Ethnopharmacol. 83: 19-24, 2002. Go to original source...
    8. Lutterodt, G.D.: Inhibition of gastrointestinal release of acetylcholine by quercetin as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal disease.-J. Ethnopharmacol. 25: 235-247, 1989. Go to original source...
    9. Mohapatra, A., Rout, G.R.: Optimization of primer screening for evaluation of genetic relationship in rose cultivars.-Biol. Plant. 50: 295-299, 2006. Go to original source...
    10. Narasimhan, S., Padmesh, P., Nair, G.M.: Assessment of genetic diversity in Coscinium fenestratum.-Biol. Plant. 50: 111-113, 2006. Go to original source...
    11. Padilla-Ramírez J.S., González-Gaona E., Esquivel-Villagrana F., Mercado-Silva E., Hernández-Delgado S., Mayek-Pérez N.: [Characterization of outstanding guava germplasm from the Calvillo-Cañ ones region, México.]-Rev. Fitotec. Mex. 25: 393-299, 2002. [In Spanish] Go to original source...
    12. Padmesh, P., Reji, J.V., Jinish Dhar, M.: Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD.-Biol Plant. 50: 367-372, 2006. Go to original source...
    13. Prakash, D.P., Narayanaswamy, P., Sondur, S.N.: Analysis of molecular diversity in guava using RAPD markers.-J. hort. Sci. Biotechnol. 77: 287-293, 2002. Go to original source...
    14. Rivera-Arce, E., Gattuso, M., Lozoya, X.: Anatomical identity parameters of the crude drug Psidii guajavae-folium.-Pharmaceut. Biol. 41: 516-521, 2003. Go to original source...
    15. Vargas-Alvarez, D., Soto-Hernández, M., González-Hernández, V.A., Engleman, E.M., Martínez-Garza, A.: [Kinetics of accumulation and distribution of flavonoids in guava (Psidium guajava L.).]-Agrociencia 40: 109-115, 2006. [In Spanish]
    16. Welsh, J., McClelland, M.: Fingerprinting genomes using PCR with arbitrary primers.-Nucleic Acids Res. 18: 7213-7218, 1990. Go to original source...
    17. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucleic Acids Res. 18: 6531-6535, 1990. Go to original source...

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