biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:129-132, 2009 | DOI: 10.1007/s10535-009-0018-y

Determination of oxalate in plant tissues with oxalate oxidase prepared from wheat

E.-E. Liu1,*, W. Luo1, H. Zhou1, X.-X. Peng1
1 Laboratory of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou, P.R. China

A method for determination of oxalate with oxalate oxidase (OxO, EC 1.2.3.4) prepared from wheat bran, is based on specific oxidation of oxalate to produce H2O2. The H2O2 formed was colorimetrically determined using horseradish peroxidase-catalyzed oxidation of 4-aminoantipyrine and N,N-dimethylaniline by H2O2. The new method was tested on rice, buckwheat, soybean and oxalis leaves, showing it is precise, sensitive, inexpensive, highly reproducible and simple to perform. Good agreement could be obtained between this method and the HPLC.

Keywords: ascorbic acid; enzymatic analysis; interference
Subjects: buckwheat; Fagopyrum esculentum; Glycine max; Oryza sativa; oxalate; oxalate oxidase; Oxalis; rice; soybean; Triticum aestivum; wheat

Received: February 16, 2007; Accepted: August 25, 2007; Published: March 1, 2009  Show citation

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Liu, E.-E., Luo, W., Zhou, H., & Peng, X.-X. (2009). Determination of oxalate in plant tissues with oxalate oxidase prepared from wheat. Biologia plantarum, 53(1), 129-132. doi: 10.1007/s10535-009-0018-y
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