Biologia plantarum 53:138-140, 2009 | DOI: 10.1007/s10535-009-0020-4
Developmental expression of β-glucosidase in olive leaves
- 1 College of Life Science, Henan Agricultural University, Zhengzhou, P.R. China
Plant β-glucosidases catalyze the hydrolysis of glycosidic linkages and play a vital role in defense against pathogens and stress. The present work investigated the relationship between leaf development and β-glucosidase protein content in Olea europea L. (cv. Picual) leaves. The total chlorophyll content increased with leaf age in current-season leaves. Immunoblot analysis revealed that the content of 61 kD protein of β-glucosidase also increased with leaf age, and that the enzyme existed in three isoforms (pI 5.8-6.2). Statistical analysis indicated a strong correlation between chlorophyll and β-glucosidase protein contents.
Keywords: chlorophyll content; immunoblot; leaf development; Olea europaea L
Subjects: chlorophyll a,b; β-glucosidase; immunoblotting; leaf ontogeny; Olea europea; olive
Received: February 7, 2007; Accepted: November 17, 2007; Published: March 1, 2009 Show citation
References
- Ciafardini, G., Zullo, B.A.: Microbiological activity in stored olive-oil.-Int. J. Food Microbiol. 75: 111-118, 2002.
Go to original source... - Konno, K., Hirayama, C., Yasui, H., Nakamura, M.: Enzymatic activation of oleuropein: a protein crosslinker used as a chemical defense in the privet tree.-PNAS 96: 9159-9164, 1999. Go to original source...
- Mazzuca, S., Spadafora, A., Innocenti, A., M.: Cell and tissue localization of β-glucosidase during the ripening of olive fruit (Olea europaea) by in situ activity assay.-Plant Sci. 171: 726-733, 2006. Go to original source...
- Melgar, J.C., Syvertsen, J.P., Martínez, V., García-Sánchez, F.: Leaf gas exchange, water relations, nutrient content and growth in citrus and olive seedlings under salinity.-Biol. Plant. 52: 385-390, 2008. Go to original source...
- Minami, Y., Kanafuji, T., Miura, K.: Purification and characterization of a β-glucosidase from Polygonum tinctorium, which catalyzes preferentially the hydrolysis of indican.-Biosci. Biotechnol. Biochem. 60: 147-149, 1996. Go to original source...
- Minami, Y., Takao, H., Kanafuji, T., Miura, K., Kondo, M., Hara-Nishimura, I., Nishimura, M., Matsubara, H.: β-Glucosidase in the indigo plant: intracellular localization and tissue specific expression in leaves.-Plant Cell Physiol. 38: 1069-1074, 1997. Go to original source...
- Mukherjee, D., Kumar, R.: Kinetin regulates plant growth and biochemical changes during maturation and senescence of leaves, flowers, and pods of Cajanus cajan L.-Biol. Plant. 51: 80-85, 2007. Go to original source...
- Pontikis, C.A., Hagidimitriou, H.: Seasonal changes in CO2 assimilation in leaves of five major Greek olive cultivars.-Sci. Hort. 104: 11-24, 2005. Go to original source...
- Poulton, E.J.: Cyanogenesis in plants.-Plant Physiol. 94: 401-405, 1990. Go to original source...
- Wang, W., Scali, M., Vignani, R., Spadafora, A., Sensi, E., Mazzuca, S., Cresti, M.: Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds.-Electrophoresis 24: 2369-2375, 2003. Go to original source...
- Wang, W., Vignani, R., Scali, M., Cresti, M.: A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis.-Electrophoresis 27: 2782-2786, 2006. Go to original source...