biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:141-144, 2009 | DOI: 10.1007/s10535-009-0021-3

In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants

M. Mingozzi1,2, S. Morini1,*
1 Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi", Sezione di Coltivazioni Arboree, Università di Pisa, Pisa, Italy
2 Dipartimento di Produzione Vegetale, Università di Milano, Milano, Italy

The effect of in vitro cultivation of donor shoots on subsequent morphogenesis in leaf explants of quince (Cydonia oblonga Mill.) clone BA29 was investigated. Proliferating donor shoots were cultured in ventilated or closed vessels under different photosynthetic photon flux densities (PPFD; 200 and 100 µmol m-2 s-1) with 0, 15, 30 g dm-3 sucrose. Shoots grown in ventilated vessels, especially with sucrose at 15 or 30 g dm-3, were better developed with fully expanded leaves compared to those in standard closed vessels. Leaves collected from pre-treated donor shoots were used to assess regeneration capacity. Somatic embryo production was highest in leaves harvested from shoots cultured in closed vessels with 30 g dm-3 sucrose and in ventilated vessels with 15 and 30 g dm-3 sucrose and under high PPFD which was, in comparison with the control treatment (closed vessel, 30 g dm-3 sucrose and low PPFD), about 2 to 2.5 times higher. A similar response was observed for root regeneration.

Keywords: ventilation; PPFD; sucrose concentration; regeneration capacity; adventitious root; somatic embryogenesis
Subjects: Cydonia oblonga; cytokinins; in vitro culture, direct regeneration; in vitro culture, micropropagation; in vitro culture, rooting; in vitro culture, somatic embryogenesis; photon flux density; sucrose; sugars

Received: February 3, 2007; Accepted: November 30, 2007; Published: March 1, 2009  Show citation

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Mingozzi, M., & Morini, S. (2009). In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants. Biologia plantarum, 53(1), 141-144. doi: 10.1007/s10535-009-0021-3
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    References

    1. Armstrong, J., Lemos, E.E.P., Zobayed, S.M.A., Justin, S., Armstrong, W.: A humidity-induced convective through flow ventilation system benefits Annona squamosa L. explants and coconut calloid.-Ann. Bot. 79: 31-40, 1997. Go to original source...
    2. Brand, M.H., Lineberger, R.D.: The effect of leaf source and developmental stage on shoot organogenic potential of sweetgum (Liquidambar styracifula L.) leaf explants.-Plant Cell Tissue Organ Cult. 24: 1-7, 1991. Go to original source...
    3. Brown, D.C.W., Thorpe, T.A.: Plant regeneration by organogenesis.-In: Vasil, I. K. (ed.): Cell Culture and Somatic Cell Genetics of Plants, Vol. 3: Plant Regeneration and Genetic Variability. Pp. 49-65. Academic Press, Orlando 1986. Go to original source...
    4. Burritt, D.J., Leung, D.W.M.: Adventitious shoot regeneration from Begonia × erythrophylla petiole sections is developmentally sensitive to light quality.-Physiol. Plant. 118: 289-296, 2003. Go to original source...
    5. Casanova, E., Moysset, L., Trillas, M.I.: Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots.-Biol. Plant. 52: 1-8, 2008. Go to original source...
    6. Chen, L., Bhagsari, A., Carter, J.: Effects of medium composition and culture duration on in vitro morphogenesis of sweet potato.-Biol. Plant. 50: 114-117, 2007. Go to original source...
    7. Cournac, L., Dimon, B., Carrier, P., Lohou, A., Chagvardieff, P.: Growth and photosynthetic characteristics of Solanum tuberosum plantlets cultivated in vitro in different conditions of aeration, sucrose supply, and CO2 enrichment.-Plant Physiol. 97: 112-117, 1991. Go to original source...
    8. Dahleen, L.S.: Donor-plant environment effects on regeneration from barley embryo-derived callus.-Crop Sci. 39: 682-685, 1999. Go to original source...
    9. D'Onofrio, C., Morini, S., Bellocchi, G.: Effect of light quality on somatic embryogenesis of quince leaves.-Plant Cell Tissue Organ Cult. 53: 91-98, 1998. Go to original source...
    10. D'Onofrio, C., Morini, S.: Simultaneous regeneration of different morphogenic structures from quince leaves as affected by growth regulator combination and treatment length.-Biol. Plant. 47: 321-325, 2003/4. Go to original source...
    11. Driver, J.A., Kuniyuki, A.H.: In vitro propagation of Paradox walnut rootstock.-HortScience 19: 507-509, 1984. Go to original source...
    12. Ekiz, H., Konzak, C.F.: Effects of light regimes on anther culture response in bread wheat.-Plant Cell Tissue Organ Cult. 50: 7-12, 1997. Go to original source...
    13. Fujiwara, K., Kozai, T.: Physical microenvironment and its effects.-In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (ed.): Automation and Environmental Control in Plant Tissue Culture. Pp. 319-369. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    14. George, E.F., Sherrington, P.D. (ed.): Plant Propagation by Tissue Culture.-Eastern Press, Reading 1984.
    15. Han, K.H., Meilan, R., Ma, C., Strauss, S.H.: An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).-Plant Cell Rep. 19: 315-320, 2000. Go to original source...
    16. Hess, J.R., Carman, J.G.: Embryogenic competence of immature wheat embryos: genotype, donor plant environment, and endogenous hormone levels.-Crop Sci. 38: 249-253, 1998. Go to original source...
    17. Jackson, M.B., Abbott, A.J., Belcher, A.R., Hall, K.C., Butler, R., Cameron, J.: Ventilation in plant tissue culture and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development.-Ann. Bot. 67: 229-237, 1991. Go to original source...
    18. Kozai, T.: Photoautotrophic micropropagation.-In Vitro cell. dev. Biol. Plant 27: 47-51, 1991. Go to original source...
    19. Kozai, T., Jeong, B.R., Kubota, C., Murai, Y.: Effects of volume and initial strength of medium on the growth, photosynthesis and ion uptake of potato (Solanum tuberosum L.) plantlet in vitro.-J. jap. Soc. hort. Sci. 64: 63-71, 1995. Go to original source...
    20. Kozai, T., Kubota, C.: Developing a photoautotrophic micropropagation system for woody plants.-J. Plant Res. 114: 525-537, 2001. Go to original source...
    21. Lucchesini, M., Monteforti, G., Mensuali-Sodi, A., Serra, G.: Leaf ultrastucture, photosynthetic rate and growth of myrtle plantlets under different in vitro culture conditions.-Biol. Plant. 50: 161-168, 2006. Go to original source...
    22. Majada J, Fal, M.A., Tadeo, F., Sanchez-Tames, R.: Effects of natural ventilation on leaf ultrastructure of Dianthus caryophyllus L. cultured in vitro.-In Vitro cell. dev. Biol. Plant 38: 272-278, 2002. Go to original source...
    23. Martin, A.B., Cuadrado, Y., Guerra, H., Gallego, P., Hita, O., Martin, L., Dorado, A., Villalobos, N.: Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L.-Plant Sci. 154: 143-151, 2000. Go to original source...
    24. Murashige, T.: Plant propagation through tissue culture.-Annu. Rev. Plant Physiol. 25: 135-166, 1874. Go to original source...
    25. Nhut, D.T., Huong, N.T.D., Van Le, B., Da Silva, J.T., Fukai, S., Tanaka, M.: The changes in shoot regeneration potential of protocorm-like bodies derived from Lilium longiflorum young stem explants exposed to medium volume, pH, light intensity and sucrose concentration pretreatment.-J. hort. Sci. Biotechnol. 77: 79-82, 2002. Go to original source...
    26. Tichá, I., Èáp, F., Pacovská, D., Hofman, P., Haisel, D., Èapková, V., Schäfer, C.: Culture on sugar medium enhances photosynthetic capacity and high light resistance of plantlets grown in vitro.-Physiol. Plant. 102: 155-162, 1998. Go to original source...

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