biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:155-158, 2009 | DOI: 10.1007/s10535-009-0024-0

Phylogenetic relationship of China Dendrobium species based on the sequence of the internal transcribed spacer of ribosomal DNA

Z.-Q. Yuan1, J.-Y. Zhang1,*, T. Liu1
1 School of Life Science, Shandong University of Technology, Zibo, P.R. China

The genetic relationship of 36 Dendrobium species in China was determined based on sequence analysis of the internal transcribed spacer (ITS) region of ribosomal DNA. Aligned sequences of the complete ITS region obtained from the 36 Dendrobium species and 2 outgroup species (Epigeneium amplum and Epigeneium nakaharaei) by using PCR amplification and direct DNA sequencing. The nrDNA ITS1 of Dendrobium was 225-234 bp and ITS2 was 239-248 bp. Phylogenetic tree was constructed, and seven main clusters were generated among the 36 Dendrobium species. From the results, D. moulmeinense was not grouped in the classification of Dendrobium and E. amplum and E. nakaharaei were shown to be divergent from Dendrobium species. The phylogenetic relationships revealed by ITS DNA analysis partially supported previously published morphological data.

Keywords: Epigeneium species; Orchidaceae; PCR; phylogenetic tree
Subjects: Dendrobium; Epigeneium; genetic diversity; internal transcribed spacer (ITS); phylogenetic tree; polymerase chain reaction (PCR)

Received: June 9, 2007; Accepted: January 5, 2008; Published: March 1, 2009  Show citation

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Yuan, Z.-Q., Zhang, J.-Y., & Liu, T. (2009). Phylogenetic relationship of China Dendrobium species based on the sequence of the internal transcribed spacer of ribosomal DNA. Biologia plantarum, 53(1), 155-158. doi: 10.1007/s10535-009-0024-0
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