biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:164-166, 2009 | DOI: 10.1007/s10535-009-0026-y

Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species

A. Akkak1,*, V. Scariot2, D. Torello Marinoni3, P. Boccacci3, C. Beltramo3, R. Botta3
1 Dipartimento Scienze Agro-Ambientali, Chimica e Difesa Vegetale, Università degli Studi di Foggia, Foggia, Italy
2 Dipartimento di Agronomia, Selvicoltura e Gestione del Territorio, Università di Torino, Grugliasco, Italy
3 Dipartimento di Colture Arboree, Università di Torino, Grugliasco, Italy

Forty one simple sequence repeats were isolated from two microsatellite enriched libraries of date palm (Phoenix dactylifera L.). After screening, 17 selected microsatellite loci were characterized and evaluated on a set of 31 cultivars and clones from Algerian and Californian germplasm. All primer pairs produced an amplification product of the expected size and detected high polymorphism among the analysed samples. These nuclear simple sequence repeat (SSR) markers are expected to be a very effective tool for evaluating genetic diversity in date palm germplasm. Acrosstaxa amplification showed the usefulness of most SSR markers in 14 other species across the genus Phoenix.

Keywords: across-taxa transferability; date palm; simple sequence repeats
Subjects: date palm; microsatellite markers; Phoenix dactylifera; simple sequence repeats (SSR)

Received: November 17, 2007; Accepted: December 6, 2008; Published: March 1, 2009  Show citation

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Akkak, A., Scariot, V., Torello Marinoni, D., Boccacci, P., Beltramo, C., & Botta, R. (2009). Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species. Biologia plantarum, 53(1), 164-166. doi: 10.1007/s10535-009-0026-y
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