biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:183-187, 2009 | DOI: 10.1007/s10535-009-0031-1

Increased cold tolerance in Arabidopsis thaliana transformed with Choristoneura fumiferana glutathione S-transferase gene

C. Huang1, T. Guo1, S. C. Zheng1, Q. L. Feng1, J. H. Liang1, L. Li1,*
1 Guangdong Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou, P.R. China

A glutathione S-transferase (GST) gene cloned from the lepidopteran spruce budworm, Choristoneura fumiferana Clem. was transformed into the model plant Arabidopsis thaliana. The CfGST-transgenic and wild-type A. thaliana were subjected to 4 and 10 °C for 48 h and their cold resistance was studied. The GST activity of the transgenic plants was 46.6 and 35.7 % higher than that of the wild-type plants after 48 h under 4 and 10 °C, respectively. Relative membrane permeability and malondialdehyde content in the transgenic plants were lower while contents of the chlorophyll and proline were higher than those in the wild-type plants under 4 and 10 °C. The survival rate of the transgenic plants was 43.7 % for 24 h under 0 °C, while survival rate of wild-type plants was 28.3 %. The results indicated that the insect GST could enhance cold resistance in the transgenic A. thaliana.

Keywords: chlorophyll; membrane permeability; malondialdehyde; proline; transgenic plants
Subjects: Arabidopsis thaliana; chlorophyll a,b; glutathione-S-transferase; malondialdehyde; membrane permeability; proline; transgenic plants

Received: July 27, 2007; Accepted: January 5, 2008; Published: March 1, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Huang, C., Guo, T., Zheng, S.C., Feng, Q.L., Liang, J.H., & Li, L. (2009). Increased cold tolerance in Arabidopsis thaliana transformed with Choristoneura fumiferana glutathione S-transferase gene. Biologia plantarum, 53(1), 183-187. doi: 10.1007/s10535-009-0031-1
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies.-Plant Soil 39: 205-207, 1973. Go to original source...
    2. Dixon, D.P., Cole, D.J., Edwards, R.: Purification, regulation and cloning of a glutathione transferase (GST) from maize resembling the auxin-inducible type-III GSTs.-Plant Biol. 36: 75-87, 1998. Go to original source...
    3. Draper, H.H., Hadley, M.: Malondialdehyde determination as index of lipid peroxidation.-Methods Enzymol. 186: 421-431, 1990. Go to original source...
    4. Feng, Q,L., Davey, K,G., Pang, S.D., Ladd, A., Retnakaran, T.R.A., Tomkins, B.L., Zheng, S., Palli, S.R.: Glutathione S-transferase from the spruce budworm, Choristoneura fumiferana: identification, characterization, localization, cDNA cloning, and expression.-Insect Biochem. mol. Biol. 29: 779-793, 1999. Go to original source...
    5. Feng, Q.L., Davey, K.G., Pang, A.S., Primavera, M., Ladd, T.R., Zheng, S.C., Sohi, S.S., Retnakaran, A., Palli, S.R.: Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Choristoneura fumiferana.-J. Insect Physiol. 47: 1-10, 2001. Go to original source...
    6. Fujita, M., Hossain, M.Z.: Modulation of pumpkin glutathione S-transferases by aldehydes and related compounds.-Plant Cell Physiol. 44: 481-490, 2003. Go to original source...
    7. Gajewska, E., Sklodowska, M., Slaba, M., Mazur, J.: Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots.-Biol. Plant. 50: 653-659, 2006 Go to original source...
    8. Grisdale, D.: An improved laboratory method for rearing large numbers of spruce budworm Choristoneura fumiferana (Lepidoptera:Tortricidae).-Can. Entomol. 102: 1111-1117, 1970. Go to original source...
    9. Hossain, M.Z., Hossain.M.D., Fujitam, M.: Induction of pumpkin glutathione S-transferases by different stresses and its possible mechanisms.-Biol. Plant. 50: 210-218, 2006 Go to original source...
    10. Irzyk, G.P., Fuerst, E.P.: Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays).-Plant Physiol. 102: 803-310, 1993. Go to original source...
    11. Izuka, M., Inoue, Y., Murata, K., Kimura, A.: Purification and some properties of glutathione S-transferase from Escherichia coli B.-J. Bacteriol. 171: 6039-6042, 1989. Go to original source...
    12. Jepson, I., Holt, D.C., Roussel, V., Wright, S.Y., Greenland, A.J.: Transgenic plant analysis as a tool for the study of maize glutathione S-transferases.-In: Hatzios. K.K. (ed.): Regulation of Enzymatic Systems Detoxifying Xenobiotics in Plants. Pp. 313-323. Kluwer Academic Publishers, Dordrecht 1997. Go to original source...
    13. Kosová, K., Práąil, I.T., Vítámvás, P.: The relationship between vernalization-and photoperiodically-regulated genes and the development of frost tolerance in wheat and barley.-Biol. Plant. 52: 601-615, 2008. Go to original source...
    14. Li, L., Pan, R.C.: Increasing yield and drought resistance of groundnut using plant growth regulators.-In: Gowda, C.L.L., Nigam, S.N., Johansen, C., Renard C. (ed.): Achieving High Groundnut Yields. Pp. 147-155. International Crops Reasearch Institute for the Semi-Arid Tropics, Patancheru 1996.
    15. MacKinney, G.: Absorption of light by chlorophyll solutions.-J. biol. Chem. 140: 315-322, 1941. Go to original source...
    16. Mannervik, B., Guthenberg, C.: Glutathione transferase (human placenta).-Methods Enzymol. 77: 231-235, 1981. Go to original source...
    17. Marrs, K.A.: The functions and regulation of glutathione S-transferases in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 127-158, 1996. Go to original source...
    18. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    19. Qi, Y.C., Zhang, S.M., Wang, L.P., Wang, M.D., Zhang, H.: Overexpression of GST accelerates the growth of transgenic Arabidopsis under the salt stress.-J. Plant Physiol. mol. Biol. 30: 517-552, 2004.
    20. Roxas, V.P., Smith, R.K., Allen, E.R., Allen, R.D.: Over-expression of glutathione S-transferase/glutathione peroxidase enhance the growth of transgenic tobacco seedlings during stress.-Natur. Biotechnol. 15: 988-991, 1997. Go to original source...
    21. Sheehan, D., Meade, G., Foley, V.M., Dowd, C.A.: Structure, function, and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily.-Biochem. J. 360: 1-16, 2001. Go to original source...
    22. Sung, D.Y.E., Vierling, E., Guy, C.L.: Comprehensive expression profile analysis of the Arabidopsis Hsp70_gene family.-Plant Physiol. 126: 789-800, 2001. Go to original source...
    23. Takesawa, T., Ito, M., Kanzaki, H., Kameya, N., Nakamura, I.: Over-expression of glutathione S-transferase in transgenic rice enhances germination and growth at low temperature.-Mol. Breed. 9: 93-101, 2002. Go to original source...
    24. Wagner, U., Edwards, R., Dixon, D.P., Mauch, F.: Probing the diversity of the Arabidopsis glutathione S-transferase gene family.-Plant mol. Biol. 49: 515-532, 2002. Go to original source...
    25. Wan, X.R., Li, L.: Molecular cloning and characterization of a dehydration-inducible cDNA encoding a putative 9-cis-epoxycarotenoid dioxygenase in Arachis hypogaea L.-DNA Seq. 16: 217-223, 2005. Go to original source...
    26. Zhou, J., Goldsbrough, P.B.: An Arabidopsis gene with homology to glutathione S-transferase is regulated by ethylene.-Plant mol. Biol. 22: 517-525, 1993. Go to original source...

    Return to the content