biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:249-256, 2009 | DOI: 10.1007/s10535-009-0047-6

Response of maize genotypes to salinity stress in relation to osmolytes and metal-ions contents, oxidative stress and antioxidant enzymes activity

J. Kholová1, R. K. Sairam1,*, R.C. Meena1, G. C. Srivastava1
1 Indian Agricultural Research Institute, Division of Plant Physiology, New Delhi, India

Effect of long term soil salinity (control-S0 and three levels S1 to S3) was studied in two maize (Zea mays L.) genotypes, PEHM 3 (comparatively tolerant) and Navjot (susceptible) at vegetative and anthesis stages during summer-rainy season. Salinity stress decreased relative water content (RWC), chlorophyll (Chl) and carotenoid (Car) contents, membrane stability index (MSI), potassium and calcium contents, and increased the contents of superoxide radical (O2 .-), hydrogen peroxide (H2O2), thiobarbituric acid reactive substances (TBARS), proline, glycinebetaine, total soluble sugars, and sodium, and Na+/K+ and Na+/Ca2+ ratios in both the genotypes. Contents of zinc, copper, manganese and iron increased up to S2. Though under S0 PEHM 3 had higher content of all the metals, Navjot recorded higher content of Zn at all salinity levels and contents of all metal ions at S2 and S3. Activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) increased upto S2 in both the genotypes, and upto S3 in PEHM 3 at the two stages. Salinity induced decrease in RWC, Chl, Car, MSI, K+ and Ca2+ was significantly greater in Navjot, which also recorded higher Na+ content and Na+/K+ and Na+/Ca2+ ratios than PEHM-3. PEHM-3 recorded higher contents of proline, glycine-betaine, total soluble sugars, K+, Ca2+, activity of SOD, APX, CAT, GR, and comparatively lower O2 .-, H2O2 and TBARS contents compared to Navjot.

Keywords: ascorbate peroxidase; calcium; catalase; glutathione reductase; glycinebetaine; heavy metals; osmolytes; potassium; proline; sodium; soluble sugars; superoxide; superoxide dismutase
Subjects: ascorbate peroxidase; calcium; carotenoids; catalase; chlorophyll a,b; copper; glutathione reductase; glycinebetaine; hydrogen peroxide; iron; maize; manganese; potassium; proline; relative water content (RWC); sodium; sugars; superoxide; superoxide dismutase (SOD); thiobarbituric acid reactive substances; Zea mays; zinc

Received: April 6, 2007; Accepted: February 20, 2008; Published: June 1, 2009  Show citation

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Kholová, J., Sairam, R.K., Meena, R.C., & Srivastava, G.C. (2009). Response of maize genotypes to salinity stress in relation to osmolytes and metal-ions contents, oxidative stress and antioxidant enzymes activity. Biologia plantarum, 53(2), 249-256. doi: 10.1007/s10535-009-0047-6
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