biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:257-262, 2009 | DOI: 10.1007/s10535-009-0048-5

The influence of cold acclimation on antioxidative enzymes and antioxidants in sensitive and tolerant barley cultivars

F. Dai1, Y. Huang2, M. Zhou3, G. Zhang1,*
1 Agronomy Department, Huajiachi Campus, Zhejiang University, Hangzhou, P.R. China
2 College of Agriculture, Guangxi University, Nanning, P.R. China
3 Tasmanian Institute of Agricultural Research, University of Tasmania, Kings Meadows, Australia

In order to better understand the role of cold acclimation in alleviating freezing injury, two barley cultivars with different cold tolerance, i.e. a sensitive cv. Chumai 1 and a tolerant cv. Mo 103, were used. The freezing treatment increased leaf soluble protein content more in the tolerant cultivar than in the sensitive one. Cold acclimation increased H2O2 content of the two cultivars during freezing treatment, especially in Mo 103. Glutathione and ascorbate contents during freezing and recovery were significantly higher in cold-acclimated plants than in non-acclimated ones. Activities of peroxidase, ascorbate peroxidase and glutathione reductase were also higher in cold-acclimated plants than non-acclimated plants during freezing treatment. However, there was no significant difference between cold-acclimated plants and the control plants in catalase activity. It may be assumed that cold acclimation induced H2O2 production, which in turn enhanced activities of antioxidative enzymes and synthesis of antioxidants, resulting in alleviation of oxidative stress caused by freezing.

Keywords: ascorbate peroxidase; catalase; glutathione reductase; Hordeum vulgare; hydrogen peroxide; low temperature; peroxidase; reactive oxygen species; recovery
Subjects: ascorbate peroxidase; ascorbic acid; barley; catalase; cold stress; freezing; glutathione; glutathione reductase; Hordeum vulgare; hydrogen peroxide; peroxidase; proteins; temperature, low

Received: July 30, 2007; Accepted: February 19, 2008; Published: June 1, 2009  Show citation

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Dai, F., Huang, Y., Zhou, M., & Zhang, G. (2009). The influence of cold acclimation on antioxidative enzymes and antioxidants in sensitive and tolerant barley cultivars. Biologia plantarum, 53(2), 257-262. doi: 10.1007/s10535-009-0048-5
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