biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:294-300, 2009 | DOI: 10.1007/s10535-009-0053-8

Dark and light green tissues of tobacco leaves systemically infected with tobacco mosaic virus

O. L. Burundukova1, M. V. Sapotsky1, A. V. Kochetov, E. A. Trifonova2, V. I. Malinovsky1,*
1 Institute of Biology and Soil Science, Vladivostok, Russia
2 Institute of Cytology and Genetics, Novosibirsk, Russia

There are significant changes in the structure of the upper tobacco (Nicotiana tabacum L.) leaves systemically infected with tobacco mosaic virus (TMV) especially in the light green tissue (LGT). Dark green areas (DGI) had intermediate status between healthy tissue and LGT. DGI contained significantly less infectious TMV and viral antigen than the LGT. The DGI, LGT and healthy tissues did not differ in the permeability of cell membranes and in the set of acidic pathogenesis-related (PR) proteins but the total content of PR-proteins in the healthy plants was higher than in the infected ones with the DGI being intermediate between healthy tissue and LGT. The crude leaf extracts from DGI and LGT showed less total ribonuclease activity and ribonuclease isozymes in comparison with control.

Keywords: Nicotiana tabacum; pathogenesis-related proteins; permeability of cell membranes; ribonuclease
Subjects: chloroplast; leaf anatomy; leaf epidermis; leaf parenchyma; Nicotiana tabacum; pathogenesis-related (PR) proteins; proteins; stomata number and size; tobacco; tobacco mosaic virus

Received: April 12, 2007; Accepted: January 20, 2008; Published: June 1, 2009  Show citation

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Burundukova, O.L., Sapotsky, M.V., Kochetov, A.V., Trifonova, E.A., & Malinovsky, V.I. (2009). Dark and light green tissues of tobacco leaves systemically infected with tobacco mosaic virus. Biologia plantarum, 53(2), 294-300. doi: 10.1007/s10535-009-0053-8
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