biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:307-311, 2009 | DOI: 10.1007/s10535-009-0055-6

Cotton somatic embryo morphology affects its conversion to plant

S. S. Hussain1,*, A. Q. Rao2, T. Husnain2, S. Riazuddin2
1 Department of Biosciences, COMSATS Institute of Information Technology, H-8/1, Islamabad, Pakistan
2 National Centre of Excellence in Molecular Biology, University of the Punjab, Thokar Niaz Baig Lahore, Pakistan

Somatic embryos differentiated from hypocotyl explant in cotton (Gossypium hirsutum L.) exhibited very divergent morphologies. Six different types of somatic embryos based on cotyledon development were observed. The growth hormones (2,4-dichlorophenoxyacetic acid and kinetin) used in induction and maintenance media did not affect embryo rooting and germination. The 95 % conversion of normal embryos (with two cotyledons) was achieved, while an overall conversion was only 38 %. Horn shaped embryos failed to exhibit shoot growth. Poorly developed apical meristems were responsible for lower conversion percentages in some of embryo classes. However, regenerated plants phenotypically resembled to seed grown control plants regardless of somatic embryo morphology.

Keywords: auxins; cytokinins; Gossypium hirsutum; micropropagation
Subjects: auxins; cotton; cytokinins; ex vitro transfer; Gossypium hirsutum; in vitro culture, somatic embryogenesis

Received: November 28, 2006; Accepted: November 11, 2007; Published: June 1, 2009  Show citation

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Hussain, S.S., Rao, A.Q., Husnain, T., & Riazuddin, S. (2009). Cotton somatic embryo morphology affects its conversion to plant. Biologia plantarum, 53(2), 307-311. doi: 10.1007/s10535-009-0055-6
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