Biologia plantarum 53:317-320, 2009 | DOI: 10.1007/s10535-009-0057-4
Genetic variability in the endemic Leucojum valentinum
- 1 Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Dpto. Botánica, Universidad de Valencia, Burjassot, Valencia, Spain
- 2 Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Jardín Botánico de Valencia, Valencia, Spain
- 3 Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Valencia, Spain
- 4 Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Valencia, Spain
The genetic variability of Leucojum valentinum Pau (Amaryllidaceae), a vulnerable endemic species restricted to a small area in the region of Valencia (Eastern Spain), has been studied using random amplified polymorphic DNA (RAPD) markers. A total of 197 individuals from eleven populations were studied using 13 RAPD primers. Our results show high variability for the species, low differentiation among populations and uncorrelated levels of genetic variability and population size. Four groups in which three populations (SAG, PUG and COL) are separated from all the others were found, but without connection to geographical location.
Keywords: conservation; fragmented populations; Mediterranean region; molecular markers; populations' management; RAPD; threatened plants
Subjects: dendrogram; genetic diversity; Leucojum valentinum; random amplified polymorphic DNA
Received: December 14, 2006; Accepted: January 12, 2008; Published: June 1, 2009 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Allphin, L., Windham, M.D., Harper, K.T.: Genetic diversity and gene flow in the endangered dwarf bear poppy, Arctomecon humilis (Papaveraceae).-Amer. J. Bot. 85: 1251-1261, 1998.
Go to original source... - Barrett, S.C.H., Kohn, J.R.: Genetic and evolutionary consequences of small population size in plants. Implications for conservation.-In: Falk, D.A., Holsinger, K.E. (ed.): Genetics and Conservation of Rare Plants. Pp. 75-86. Oxford University Press, New York 1991.
- Cenis, J.L.: Rapid extraction of fungal DNA for PCR amplification.-Nucl. Acids Res. 20: 2380, 1992.
Go to original source... - Cotrim, H.C., Chase, M.W., Pais, M.S.: Silene rothmaleri P. Silva (Caryophyllaceae), a rare, fragmented but genetically diverse species.-Biodivers. Conserv. 12: 1083-1098, 2003.
Go to original source... - Domínguez, L.F., Galicia, H.D., Moreno, R.L., Moreno, S.J.C., Sainz, O.H.: Threatened plants in the peninsular and balearic Spain: a report based on the EU habitats directive.-Biol. Conserv. 76: 123-133, 1996.
Go to original source... - Ellstrand, N.C., Elam, D.R.: Population genetic consequences of small population size: implications for plant conservation.-Annu. Rev. Ecol. Syst. 24: 217-242, 1993.
Go to original source... - Excoffier, L., Smouse, P.E., Quattro, J.M.: Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.-Genetics 131: 479-491, 1992.
Go to original source... - Fenster, C.B., Dudash, M.R.: Genetic considerations for plant restoration and conservation.-In: Bowles, M.L., Whelan, C.J. (ed.): Restoration of Endangered Species: Conceptual Issues, Planning, and Implementation. Pp. 34-62. Cambridge University Press, Cambridge 1994.
Go to original source... - Hamrick, J.L., Linhart, Y.B., Mitton, J.B.: Relationships between life history characteristics and electrophoretically detectable genetic variation in plants.-Annu. Rev. Ecol. Syst. 10: 173-200, 1979.
Go to original source... - Knapp, E.E., Rice, K.J.: Genetic structure and gene flow in Elymus glaucus (blue wildrye): implications for native grassland research.-Restor. Ecol. 4: 1-10, 1996.
Go to original source... - Mohapatra, A., Rout, G.R. Optimization of primer screening for evaluation of genetic relationships in rose cultivars.-Biol. Plant. 50: 295-299, 2006.
Go to original source... - Narasimhan, S., Padmesh, P., Nair, G.M. Assessment of genetic diversity in Coscinium fenestratum.-Biol. Plant. 50: 111-113, 2006.
Go to original source... - Nei, M.: Estimation of average heterozygosity and genetic distance from a small number of individuals.-Genetics 89: 583-590, 1978.
Go to original source... - Norusis, M.J.: SPSS Base System User's Guide.-SPSS Inc., Chicago 2002.
- Nybom, H., Bartish, I.: Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants.-Perspect. Plant Ecol. Evol. Syst. 3: 92-114, 2000.
Go to original source... - Padmesh, P., Reji, J.V., Jinish Dhar, M., Seeni, S.: Estimation of genetic diversity in varieties of Mucuna pruriensis using RAPD.-Biol. Plant. 50: 367-372, 2006.
Go to original source... - Refoufi, A., Esnault, M.A.: Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data.-Biol. Plant. 52: 543-547, 2008.
Go to original source... - Rohlf, F.J.: NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Setauket. Version 2.22.-Exeter Software, New York 2004.
- Rout, G.R.: Evaluation of genetic relationships in Typhonium species through random amplified polymorphic DNA markers.-Biol. Plant. 50: 127-130, 2006.
Go to original source... - Saunders, D.A., Hobbs, R.J., Margules, C.R.: Biological consequences of ecosystem fragmentation: a review.-Conserv. Biol. 5: 18-32, 1991.
Go to original source... - Schneider, S., Roessli, D., Excoffier, L.: Arlequin V. 2.00: a Software for Population Genetics Data Analysis.-Genetics and Biometry Laboratory, University of Geneva, Switzerland 2000.
- Segarra-Moragues, J.G., Catalán, P.: Life history variation between species of the relictual genus Borderea (Dioscoreaceae): phylogeography, genetic diversity and population genetic structure assessed by RAPD markers.-Biol. J. Linn. Soc. 80: 483-498, 2003.
Go to original source... - Wroblewska, A., Brzosko, E., Czarnecka, B., Nowosielski, J.: High levels of genetic diversity in populations of Iris aphylla L. (Iridaceae), an endangered species in Poland.-Bot. J. Linn. Soc. 142: 65-72, 2003.
Go to original source... - Xena de Enrech, N.: [A decade of application of the RAPD method: Achievements and shortcomings in the study of genetic relationships in plants]-Acta cient. venezol. 51: 197-206, 2000. [In Spanish]
- Young, A., Boyle, T., Brown, T.: The population genetic consequences of habitat fragmentation for plants.-Trends Ecol. Evol. 11: 413-418, 1996.
Go to original source... - Zeng, J., Zou, Y.P., Bai, J.Y., Zheng, H.S.: RAPD analysis of genetic variation in natural populations of Betula alnoides from Guangxi, China.-Euphytica 134: 33-41, 2003.
Go to original source...



