biologia plantarum

Biologia plantarum 53:347-350, 2009 | DOI: 10.1007/s10535-009-0064-5

Micropropagation of Bixa orellana using phytohormones and triacontanol

R. Parimalan¹, P. Giridhar^1,*, H. B. Gururaj¹, G. A. Ravishankar¹

¹ Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore, India

An efficient micropropagation protocol for annatto (Bixa orellana L.) was achieved using nodal shoot tip explants. Shoot buds were obtained on the Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of indole-3-acetic acid (IAA), N⁶-benzyladenine (BA) and triacontanol (TRIA). Maximum of 213 shoot buds along with 18 primary shoots were produced on MS medium containing 0.05 µM IAA, 8.87 µM BA, and 11.2 µM TRIA. The primary shoots elongated best on MS medium containing 6.66 µM BA and 2.45 µM indole-3-butyric acid (IBA). The regenerated shoots rooted best on MS medium supplemented with 4.9 µM IBA. The in vitro rooted plantlets were hardened and establishment rate under field conditions was 70 to 80 %.

Keywords: annatto; callus; ex vitro transfer; multiple shoots; organogenesis

Subjects: auxins; Bixa orellana; cytokinins; ex vitro transfer; in vitro culture, direct regeneration; in vitro culture, micropropagation; in vitro culture, rooting; triacontanol

Received: August 9, 2007; Accepted: April 17, 2008; Published: June 1, 2009  Show citation