biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:395-399, 2009 | DOI: 10.1007/s10535-009-0075-2

In vitro organogenesis of Citrus volkameriana and Citrus aurantium

E. C. R. Tavano1, L. C. L. Stipp2, F. R. Muniz1, F. A. A. Mourão Filho1, B. M. J. Mendes2,*
1 Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil
2 Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba SP, Brazil

In vitro organogenesis of Citrus volkameriana and C. aurantium was studied considering three explant types: epicotyl segment, internodal segment, and hypocotyl segment with attached cotyledon fragment. The explants were cultured in medium according to Grosser and Gmitter (EME) supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg dm- 3 6-benzyl-aminopurine (BAP), incubated firstly in darkness for 4 weeks, and then transferred to 16-h photoperiod for 2 weeks. Comparing epicotyl and internodal segments, a higher percentage of responsive explants and a higher number of shoots per explant were obtained with epicotyl segments, regardless of the BAP concentration. For C. volkameriana the highest percentage of responsive epicotyl segments (42 %) was obtained in EME with 1.0 mg dm-3 BAP, while for C. aurantium (59 %) in EME with 0.5 mg dm-3 BAP. The organogenesis efficiency was the best with the use of the hypocotyl segment with attached cotyledon fragment (77 % for C. volkameriana and to 75 % for C. aurantium). With this explant the morphogenesis occurred only in the hypocotyl region. The in vitro organogenesis was characterized by histological analyses showing that the morphogenic process started in the cambium region near the explant cut end.

Keywords: adventitious bud; BAP; epicotyl; hypocotyl; internodal segment; sour orange; volkamer lemon
Subjects: auxins; Citrus aurantium; Citrus volkamericana; cytokinins; in vitro culture, direct regeneration

Received: August 16, 2008; Accepted: April 15, 2009; Published: June 1, 2009  Show citation

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Tavano, E.C.R., Stipp, L.C.L., Muniz, F.R., Mourão Filho, F.A.A., & Mendes, B.M.J. (2009). In vitro organogenesis of Citrus volkameriana and Citrus aurantium. Biologia plantarum, 53(2), 395-399. doi: 10.1007/s10535-009-0075-2
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    References

    1. Almeida, W.A.B., Mourão Filho, F.A.A., Mendes, B.M.J., Rodriguez, A.P.M.: Histological characterization of in vitro adventitious organogenesis in Citrus sinensis.-Biol. Plant. 50: 321-325, 2006. Go to original source...
    2. Ananthakrishnan, G., Xia, X., Elman, C., Singer, S., Paris, H.S., Gal-On, A., Gaba, V.: Shoot production in squash (Cucurbita pepo) by in vitro organogenesis.-Plant Cell Rep. 21: 739-746, 2003. Go to original source...
    3. Azevedo, F.A., Mourão Filho, F.A.A., Mendes, B.M.J., Almeida, W.A.B., Schinor, E.H., Pio, R., Barbosa, J.M., Guidetti-Gonzalez, S., Carrer, H., Lam, E.: Genetic transformation of rangpur lime (Citrus limonia Osbeck) with the bO (bacteriopsin) gene and its initial evaluation for Phytophthora nicotianae resistance.-Plant mol. Biol. Rep. 24: 1-12, 2006. Go to original source...
    4. Bordón, Y., Guardiola, J.L., García-Luis, A.: Genotype affects the morphogenic response in vitro of epicotyl segments of Citrus rootstocks.-Ann. Bot. 86: 159-166, 2000. Go to original source...
    5. Boscariol, R.L., Monteiro, M., Takahashi, E.K., Chabregas, S.M., Vieira, M.L.C., Vieira, L.G.E., Pereira, L.F.P., Mourão Filho, F.A.A., Cardoso, S.C., Christiano, R.S.C., Bergamin Filho, A., Barbosa, J.M., Azevedo, F.A., Mendes, B.M.J.: Attacin A gene from Tricloplusia ni reduces susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis 'Hamlin'.-J. amer. Soc. hort. Sci. 131: 530-536, 2006. Go to original source...
    6. Cervera, C., Navarro, A., Navarro, L., Peña, L.: Production of transgenic adult plants from Clementine mandarin by enhancing cell competence for transformation and regeneration.-Tree Physiol. 28: 55-66, 2008. Go to original source...
    7. Chen, R., Namimatsu, S., Nakadozono, Y., Bamba, T., Nakazawa, Y., Gyokusen, K.: Efficient regeneration of Eucommia ulmoides from hypocotyl explant.-Biol. Plant. 52: 713-717, 2008. Go to original source...
    8. Costa, M.G.C., Alves, V.S., Lani, E.R.G., Mosquim, P.R., Carvalho, C.R., Otoni, W.C.: Morphogenic gradients of adventitious bud and shoot regeneration in epicotyl explants of Citrus.-Sci. Hort. 100: 63-74, 2004. Go to original source...
    9. Divya, K., Swathi Anuradha, T., Jami, S.K., Kirti, P.B.: Efficient regeneration from hypocotyl explants in three cotton cultivars.-Biol. Plant. 52: 201-208, 2008. Go to original source...
    10. Duran-Vila, N., Gogorcena, Y., Ortega, V., Ortiz, J., Navarro, L.: Morphogenesis and tissue culture of sweet orange (Citrus sinensis (L.) Osb.): effect of temperature and photosynthetic radiation.-Plant Cell Tissue Organ Cult. 29: 11-18, 1992. Go to original source...
    11. Fernando, J.A., Vieira, M.L.C., Machado, S.R., Appezzato-da-Glória, B.: New insights into the in vitro organogenesis process: the case of Passiflora.-Plant Cell Tissue Organ Cult. 91: 37-44, 2007. Go to original source...
    12. García-Luis A., Bordón, Y., Moreira-Dias, J.M., Molina, R.V., Guardiola, J.L.: Explant orientation and polarity determine the morphogenic response of epicotyl segments of Troyer citrange.-Ann. Bot. 84: 715-723, 1999. Go to original source...
    13. García-Luis, A., Molina, R.V., Varona, V., Castelló, S., Guardiola J.L.: The influence of explant orientation and contact with the medium on the pathway of shoot regeneration in vitro in epicotyl cuttings of Troyer citrange.-Plant Cell Tissue Organ Cult. 85: 137-144, 2006. Go to original source...
    14. Grosser, J.W., Gmitter, F.G., Jr.: Protoplast fusion and citrus improvement.-Plant Breed. Rev. 8: 339-374, 1990. Go to original source...
    15. Gutiérrez-E., M.A., Luth, D., Moore, G.A.: Factors affecting Agrobacterium-mediated transformation in citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat gene of Citrus tristeza virus.-Plant Cell Rep. 16: 745-753, 1997. Go to original source...
    16. Kobayashi, A., Uchimiya, H.: Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer.-Jap. J. Genet. 64: 91-97, 1989. Go to original source...
    17. Kochba, J., Spiegel-Roy, P., Safran, H.: Adventive plants from ovules and nucelli in Citrus.-Planta 106: 237-245, 1972. Go to original source...
    18. Molina, R.V., Castelló, S., García-Luis, A., Guardiola, J.L.: Light-cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro.-Plant Cell Tissue Organ Cult. 89: 131-140, 2007. Go to original source...
    19. Moreira-Dias, J.M., Molina, R.V., Bordón, Y., Guardiola, J.L., García-Luis, A.: Direct and indirect shoot organogenic pathways in epicotyl cuttings of Troyer citrange differ in hormone requirements and in their response to light.-Ann. Bot. 85: 103-110, 2000. Go to original source...
    20. Moreira-Dias, J.M., Molina, R.V., Guardiola, J.L., García-Luis, A.: Day length and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange.-Sci. Hort. 87: 275-290, 2001. Go to original source...
    21. Moura, T.L., Almeida, W.A.B, Mendes, B.M.J., Mourão Filho, F.A.A.: [Citrus in vitro organogenesis related to BAP concentrations and explant section.]-Rev. Bras. Fruticult. 23: 240-245, 2001. [In Portuguese] Go to original source...
    22. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    23. Murashige, T., Tucker, D.P.H.: Growth factor requirements of Citrus tissue culture.-In: Chapman, H.D. (ed.): Proceedings of First International Citrus Symposium. Pp. 1155-1161. University of California, Riverside 1969.
    24. Peña, L., Cervera, M., Juarez, J., Ortega, C, Pina, J.A., Durán-Vila, N., Navarro, L.: High efficiency Agrobacterium-mediated transformation and regeneration of citrus.-Plant Sci. 104: 183-191, 1995. Go to original source...
    25. Peña, L., Martín-Tillo, M., Juárez, J., Pina, J.A., Navarro, L., Martínez-Zapater, M.: Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time.-Nature 19: 263-267, 2001. Go to original source...
    26. Ranga Swamy, N.S.: Culture of nucellar of Citrus in vitro.-Experientia 14: 111-112, 1958. Go to original source...
    27. Silva, R.P., Souza, E.S., Rebouças, F.S., Almeida, W.A.B.: [Optimization of protocols for in vitro regeneration of 'Cleópatra' (Citrus reshni Hort. ex Tan.) mandarin plants.]-Rev. Bras. Fruticult. 27: 484-487, 2005. [In Portuguese] Go to original source...
    28. Yu, C., Huang, S., Chen, C., Deng, Z., Ling, P., Gmitter, F.G., Jr.: Factors affecting Agrobacterium-mediated transformation and regeneration of sweet orange and citrange.-Plant Cell Tissue Organ Cult. 71: 147-155, 2002. Go to original source...

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