biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:461-467, 2009 | DOI: 10.1007/s10535-009-0086-z

Plasma membrane localization of soybean matrix metalloproteinase differentially induced by senescence and abiotic stress

C.-W. Cho1, E. Chung1, K. Kim1, H.-A. Soh1, Y. K. Jeong1, S.-W. Lee1, Y.-C. Lee1, K.-S. Kim1, Y.-S. Chung1, J.-H. Lee1,*
1 BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University, Busan, Republic of Korea

We isolated and characterized a stress-inducible gene, designated as Slti114, encoding matrix metalloproteinase (MMP) in soybean. The derived amino acid sequences of Slti114 show the 69 % homology with MMP2 from Glycine max (AAL27029). The size of the full-length cDNA of Slti114 is 1194 bp with open reading frame comprised of 394 amino acids. RNA expression of Slti114 was induced by low temperature or wounding. During early stage, Slti114 RNA level was extremely high, but Slti114 RNA was not detectable just after cotyledons became yellowish. Green fluorescent protein fusion expression system confirmed that Slti114-smGFP and H+-ATPase-RFP were co-localized to the plasma membrane. Purified glutathione-S-transferase (GST)-Slti114 protein was shown to digest myelin basic protein (MBP) in vitro, but not gelatin. This report provides strong evidence that plasma membrane MMP, Slti114 protein may play a critical role during abiotic stress and senescence in plant.

Keywords: abiotic stress; Glycine max; low temperature; RNA expression
Subjects: dendrogram; gene expression; Glycine max; metalloproteinase; Nicotiana benthamiana; plasma membrane; senescence; soybean; temperature, low; transgenic plants; wounding

Received: November 3, 2007; Accepted: April 27, 2008; Published: September 1, 2009  Show citation

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Cho, C.-W., Chung, E., Kim, K., Soh, H.-A., Jeong, Y.K., Lee, S.-W., ... Lee, J.-H. (2009). Plasma membrane localization of soybean matrix metalloproteinase differentially induced by senescence and abiotic stress. Biologia plantarum, 53(3), 461-467. doi: 10.1007/s10535-009-0086-z
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