biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:468-474, 2009 | DOI: 10.1007/s10535-009-0087-y

Apomeiotic pollen mother cell development in the apomictic Boechera species

K. M. Taskin1,*, K. Turgut2, R. J. Scott3
1 Department of Biology, Canakkale Onsekiz Mart University, Canakkale, Turkey
2 Department of Field Crops, Akdeniz University, Antalya, Turkey
3 Department of Biology and Biochemistry, University of Bath, Avon, England

Pollen mother cell (PMC) development in the apomictic Boechera species B. holboellii, B. gunnisoniana and B. divaricarpa were investigated by various cytological methods. In prophase I, in triploid species B. holboellii and B. gunnisoniana the individual chromosomes condensed into long strands within the nucleus. Then, in metaphase I, each PMC formed a restitutional nucleus thereby bypassing the rest of the first meiotic division. This is interpreted as representing apomeiosis. Subsequently, the restitution nuclei underwent a single cytokinesis as evidenced by the production of dyads. The cells within each dyad were separated by a callose wall. Most of the PMC in B. holboellii and B. gunnisoniana produced dyads, but a small proportion generated conspicuous tetrads. In contrast, diploid apomict B. divaricarpa produced only tetrads by simultaneous cytokinesis.

Keywords: apomixis; endosperm; male gametes
Subjects: apomixis; Boechera divaricarpa; Boechera gunnisoniana; Boechera holboellii; endosperm; male gametophyte development; ploidy; pollen mother cells

Received: March 11, 2008; Accepted: July 28, 2008; Published: September 1, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Taskin, K.M., Turgut, K., & Scott, R.J. (2009). Apomeiotic pollen mother cell development in the apomictic Boechera species. Biologia plantarum, 53(3), 468-474. doi: 10.1007/s10535-009-0087-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bicknell, R.A., Koltunow, A.M.: Understanding apomixis: recent advances and remaining conundrums. - Plant Cell 16(Suppl.): S228-S245, 2004. Go to original source...
    2. Böcher, T.: Cytological and embryological studies in the amphiapomictic Arabis holboellii complex. - Biol. Skrifter 6: 1-58, 1951.
    3. Carman, J.G.: Asynchronous expression of duplicate genes in angiosperms may cause apomixis, bispory, tetraspory, and polyembryony. - Biol. J. Linn. Soc. 61: 51-94, 1997. Go to original source...
    4. Grimanelli, D., Leblanc, O., Perotti, E., Grossniklaus, U.: Developmental genetics of gametophytic apomixis. - Trends Genet. 17: 597-604, 2001. Go to original source...
    5. Haig, D., Westoby, M.: Parent-specific gene expression and the triploid endosperm. - Amer. Natur. 134: 147-155, 1989. Go to original source...
    6. Kantama, L., Sharbel, T.F., Schranz, M.E., Mitchell-Olds, T., Vries, S., Jong, H.D.: Diploid apomicts of the Boechera holboellii complex display large-scale chromosome substitutions and aberrant chromosomes. - Proc. nat. Acad. Sci. USA 104: 14026-14031, 2007. Go to original source...
    7. Koch, M.A., Dobes, C., Mitchell-Olds, T.: Multiple hybrid formation in natural populations: concerted evolution of the internal transcribed spacer of nuclear ribosomal DNA (ITS) in north American Arabis divaricarpa (Brassicaceae). - Mol. Biol. Evol. 20: 338-350, 2003. Go to original source...
    8. Krahulcová, A., Suda, J.: A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels. - Biol Plant. 50: 457-460, 2006. Go to original source...
    9. Lin, B.-Y.: Ploidy barrier to endosperm development in maize. Genetics 107: 103-115 1984. Go to original source...
    10. Matzk, F., Meister, A., Brutovska, R., Schubert, I.: Reconstruction of reproductive diversity in Hypericum perforatum L. opens novel strategies to manage apomixis. - Plant J. 26: 275-282, 2001. Go to original source...
    11. Matzk, F., Meister, A., Schubert, I.: An efficient screen for reproductive pathways using mature seeds of monocots and dicots. - Plant J. 21: 97-108, 2000. Go to original source...
    12. Naumova, T.N., Van der Laak, J., Osadtchiy, J., Matzk, F., Kravtchenko, A., Bergervoet, J., Ramulu, K.S., Boutilier, K.: Reproductive development in apomictic populations of Arabis holboellii (Brassicaceae). - Sex. Plant Reprod. 14: 195-200, 2001. Go to original source...
    13. Noyes, R.D.: Inheritance of apomeiosis (diplospory) in fleabanes (Erigeron, Asteraceae). - Heredity 94: 193-198, 2005. Go to original source...
    14. Ozias-Akins, P., Akiyama, Y., Hanna, W.W.: Molecular characterization of the genomic region linked with apomixis in Pennisetum/Cenchrus. - Funct. Integr. Genomics 3: 94-104, 2003. Go to original source...
    15. Roy, B.A.: The breeding systems of 6 species of Arabis (Brassicaceae). - Amer. J. Bot. 82: 869-877, 1995. Go to original source...
    16. Roy, B.A., Rieseberg, L.H.: Evidence for apomixis in Arabis. - J. Hered. 80: 506-508, 1989.
    17. Rutishauser, A.: Die Entwicklungserregug des Endosperms bei pseudogamen Ranunculusarten. - Mitt. Naturforsch. Gesellsch. Schaffhausen 25: 1-45, 1954.
    18. Schranz, M.E., Dobes, C., Koch, M.A., Mitchell-Olds, T.: Sexual reproduction, hybridization, apomixis, and polyploidization in the genus Boechera (Brassicaceae). - Amer. J. Bot. 92: 1797-1810, 2005. Go to original source...
    19. Schranz, M.E., Kantama, L., De Jong, H., Mitchell-Olds, T.: Asexual reproduction in a close relative of Arabidopsis: a genetic investigation of apornixis in Boechera (Brassicaceae). - New Phytol. 171: 425-438, 2006. Go to original source...
    20. Scott, R., Dagless, E., Hodge, R., Paul, W., Soufleri, I., and Draper, J.: Patterns of gene-expression in developing anthers of Brassica napus. - Plant mol. Biol. 17: 195-207, 1991. Go to original source...
    21. Scott, R.J., Spielman, M., Dickinson, H.G.: Stamen structure and function. - Plant Cell 16: S46-S60, 2004. Go to original source...
    22. Sharbel, T.F., Mitchell-Olds, T.M., Dobes, C., Kantama, L., and de Jong, H.: Biogeographic distribution of polyploidy and B chromosomes in the apomictic Boechera holboellii complex. - Cytogenet. Genome Res. 109: 283-292, 2005. Go to original source...
    23. Sharbel, T.F., Voigt, M.L., Mitchell-Olds, T., Kantama, L., De Jong, H.: Is the aneuploid chromosome in an apomictic Boechera holboellii a genuine B chromosome? - Cytogenet. Genome Res. 106: 173-183, 2004. Go to original source...
    24. Spielman, M., Preuss, D., Li, F.L., Browne, W.E., Scott, R.J., Dickinson, H.G.: TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana. - Development 124: 2645-2657, 1997. Go to original source...
    25. Taskin, K.M., Turgut, K., Scott, R.J.: Apomictic development in Arabis gunnisoniana. - Israel J. Plant Sci. 52: 155-160, 2004. Go to original source...
    26. Vinkenoog, R., Bushell, C., Spielman, M., Adams, S., Dickinson, H.G., Scott, R.J.: Genomic imprinting and endosperm development in flowering plants. - Mol. Biotechnol. 25: 149-184, 2003. Go to original source...

    Return to the content