biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:569-572, 2009 | DOI: 10.1007/s10535-009-0103-2

Rapid in vitro propagation of Holarrhena antidysenterica using seedling cotyledonary nodes

K. Mallikarjuna1,2, G. Rajendrudu1
1 Department of Botany, Sri Venkateswara University, Tirupati, India
2 Department of Botany, Acharya Nagarjuna University, Nagarjuna Nagar, India

A rapid in vitro propagation of Holarrhena antidysenterica has been developed. Seedling cotyledonary nodes on Murashige and Skoog medium (MS) containing 2 mg dm-3 N6-benzyladenine (BA) produced highest number of multiple shoots. The shoot numbers were increased further upon subculture on MS medium supplemented with 0.5 mg dm-3 BA. By repeated subculture of derived shoots, a high multiplication rate was established. The excised shoots were rooted on MS basal medium without growth regulators. The in vitro formed shoots were also rooted ex vitro by dipping them in 2 mg dm-3 of indole-3-butyric acid (IBA) solution for 2 min before transferring them onto the hardening medium. Successful hardening and further establishment (survival 90 %) of micropropagated plants under natural conditions was observed.

Keywords: benzyladenine; ex vitro rooting; indole-3-butyric acid; micropropagation
Subjects: auxins; cytokinins; ex vitro transfer; Holarrhena antidysenterica; in vitro culture, direct regeneration

Received: July 30, 2007; Accepted: December 21, 2008; Published: September 1, 2009  Show citation

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Mallikarjuna, K., & Rajendrudu, G. (2009). Rapid in vitro propagation of Holarrhena antidysenterica using seedling cotyledonary nodes. Biologia plantarum, 53(3), 569-572. doi: 10.1007/s10535-009-0103-2
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