biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:573-577, 2009 | DOI: 10.1007/s10535-009-0104-1

Secondary embryogenesis and transient expression of the β-glucuronidase gene in hypocotyls of rapeseed microspore-derived embryos

M. R. Abdollahi1, A. Moieni1, A. H. Salmanian2, A. Mousavi2,*
1 Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran
2 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Secondary embryogenesis from rapeseed microspore-derived embryos (MDEs) was studied in three Brassica napus L. cultivars Global, PF704 and Option. The best results in terms of secondary embryogenesis percentage obtained in cultures of Global and PF704 MDEs (75.88 and 65.97 %, respectively) and PF704 produced the highest number of secondary embryos per each primary embryo (14.91 ± 2.18). After optimization of physical parameters, rapeseed hypocotyls of MDEs were bombarded with microcarriers coated with a plasmid containing GUS reporter gene. The highest levels of transient GUS expression were obtained using bombardment with gold particles of 1.6 µm, at helium pressure of 9.3 MPa, a bombardment distance of 9 cm, chamber vacuum pressure of 7.1 × 10-6 kPa and single bombardment in bombardment medium containing 0.4 M mannitol.

Keywords: anther culture; biolistic transformation; doubled haploid; gene transfer
Subjects: Brassica napus; β-glucuronidase; in vitro culture, somatic embryogenesis; particle bombardment; rapeseed; transgenic plants

Received: April 12, 2007; Accepted: December 15, 2007; Published: September 1, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Abdollahi, M.R., Moieni, A., Salmanian, A.H., & Mousavi, A. (2009). Secondary embryogenesis and transient expression of the β-glucuronidase gene in hypocotyls of rapeseed microspore-derived embryos. Biologia plantarum, 53(3), 573-577. doi: 10.1007/s10535-009-0104-1
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Banerjee, A.K., Agrawal, D.C., Nalawade S.M., Krishnamurthy, K.V.: Transient expression of β-glucuronidase in embryo axes of cotton by Agrobacterium and particle bombardment methods. - Biol. Plant. 45: 359-365, 2002. Go to original source...
    2. Chen, J.L., Beversdorf, W.D.: A combined use of microprojectile bombardment and DNA imbibition enhances transformation frequency of canola. - Theor. appl. Genet. 88: 187-192, 1994. Go to original source...
    3. Clemente, T.E., Robertson, D., Isleib, T.G., Beute, M.K., Weissinger, A.K.: Evaluation of peanut (Arachis hypogaea L.) leaflets from mature zygotic embryos as recipient tissue for biolistic gene transfer. - Transgenic Res. 1: 275-284, 1992. Go to original source...
    4. Fletcher, R., Coventry, J., Kott, L.S.: Double Haploid Technology for Spring and Winter Brassica napus. - OAC Publication, Ontario 1998.
    5. Folling, L., Olesen, A.: Transformation of wheat (Triticum aestivum L.) microspore-derived callus and microspores by particle bombardment. - Plant Cell Rep. 20: 629-636, 2001. Go to original source...
    6. Gamborg, O.L., Miller, R.A., Ojiwa, K.: Nutrient requirements of suspension culture of soybean root callus. - Exp. Cell Res. 50: 151-158, 1968. Go to original source...
    7. Hebert, D., Kikkert, J.R., Smith, F.D., Reisch, B.I.: Optimization of biolistic transformation of embryogenic grape cell suspensions. - Plant Cell Rep. 12: 585-589, 1993. Go to original source...
    8. Huang, B.: Genetic manipulation of microspores and microspore-derived embryos. - In vitro cell dev. Biol. Plant 28: 53-58, 1992. Go to original source...
    9. Janna, O.A., Maziah, M., Ahmad Parveez, G.K.A., Saleh, K.: Factors affecting delivery and transient expression of β-glucuronidase gene in Dendrobium Sonia protocorm-likebody. - Afr. J. Biotechnol. 5: 88-94, 2006.
    10. Jones-Villeneuve, E., Huang, B., Prudhomme, I., Bird, S., Kemble, R., Hattori, J., Miki, B.: Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed. - Plant Cell Tissue Organ Cult. 40: 97-100, 1995. Go to original source...
    11. Kott, L., Coventry, J.: Manual for Microspore Culture Technique for Brassica napus. - Crop Science Department, University of Guelph, Guelph 1988.
    12. Leob, T.A., Reynolds, T.L.: Transient expression of the uid A gene in pollen embryoids of wheat following microprojectile bombardment. - Plant Sci. 104: 81-91, 1994. Go to original source...
    13. Lichter, R.: Efficient yield of embryoids by culture of isolated microspore of different Brassicaceae species. - Plant Breed. 103: 119-123, 1982. Go to original source...
    14. McCabe, D., Christou, P.: Direct DNA transfer using electric discharge particle acceleration (ACCELL™ technology). - Plant Cell Tissue Organ Cult. 33: 227-236, 1993. Go to original source...
    15. Nehlin, L., Mollers, C., Bergman, P., Glimelius, K.: Transient β-glucuronidase and gfp gene expression and viability analysis of microprojectile bombarded microspores of Brassica napus L. - J. Plant Physiol. 156:175-183, 2000. Go to original source...
    16. Nehlin, L., Mollers, C., Glimelius, K.: Induction of secondary embryogenesis in MDEs of Brassica napus L. - Plant Sci. 111: 219-227, 1995. Go to original source...
    17. Parveez, G.K.A., Chowdhury, M.K.U., Saleh, N.M.: Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineesis Jacq.) using the biolistic device. - Ind. Crops Products 6: 41-50, 1997. Go to original source...
    18. Pechan, P.M., Keller, W.A., Mandy, F., Bergeron, M.: Selection of Brassica napus L. embryogenic microspores by flow sorting. - Plant Cell Rep. 7: 396-398, 1988. Go to original source...
    19. Pechan, P.M.: Successful cocultivation of Brassica napus microspores and proembryos with Agrobacterium. - Plant Cell Rep. 8: 387-390, 1989. Go to original source...
    20. Sanford, J., Smith, F.D., Russel, J.A.: Optimizing the biolistic process for different biological applications. - Methods Enzymol. 217: 483-505, 1993. Go to original source...
    21. Sangwan, R.S., Dubois, F., Ducrocq, C., Bourgeois, Y., Vilcot, B., Pawlicki, N., Sangwan-Norreel, B.S.: The embryo as a tool for genetic engineering in higher plants. - In: Terzi, M., Cella, R., Falavigna, A. (ed.): Proceedings of the VIIIth International Congress on Plant Tissue And Cell Culture. Pp. 271-277. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    22. Shu, W., Loh, C.S.: Secondary embryogenesis from thin cell layers of Brassica napus spp. oleifera. - New Phytol. 119: 427-432, 1991. Go to original source...
    23. Takahashi, N. (ed.): Chemistry of Plant Hormones. - CRC Press, Boca Raton 1986.
    24. Telmer, C.A., Simmonds, D.H., Newcomb, W.: Determination of developmental stage to obtain high frequencies of embryogenic microspores in Brassica napus. - Physiol. Plant. 84: 417-424, 1992. Go to original source...
    25. Uzelac, B., Ninkoviĉ, S., Smigocki, A., Budimir, S.: Origin and development of secondary somatic embryos in transformed embryogenic cultures of Medicago sativa. - Biol. Plant. 51: 1-6, 2007. Go to original source...

    Return to the content