biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:593-596, 2009 | DOI: 10.1007/s10535-009-0108-x

Chloroplast DNA polymorphism in different types of cytoplasmic male sterile rice

L. -J. Ou1,2, G. -W. Huang1,3, W. -J. Li1, G. P. Kang1, J. -L. Chen1, S. Luan1, L. -B. Chen1,*
1 College of Life Science, Hunan Normal University, Changsha Hunan, P.R. China
2 Key laboratory of Hunan Province for Study and Utilization of Ethnica Medicinal Plant Resources, Huaihua University, Huaihua Hunan, P.R. China
3 Department of Life Sciences, Hunan University of Science and Engineering, Yongzhou Hunan, P.R. China

The lengths of open reading frame (ORF)100 and ORF29-TrnCGCA, the intronic sequence of rps16 and the transcribed spacer of TrnTUGU-TrnLUAA in chloroplast from different lines of cytoplasmic male sterility (CMS) rice were studied using indica types, japonica types and common wild rice as controls. The results show that the lengths of ORF100 and ORF29-TrnCGCA in CMS lines are similar to those of typical indica. The sequences of the rps16 intron and the TrnTUGU-TrnLUAA spacer in sporophyte sterile types (wild-abortive type, Yinshui type and K type) are almost the same, and they also share a molecular marker of GTTGAG at nucleotide positions 220-225 in the rps16 intron. Therefore, it is speculated that the source of these three types is the same. In contrast, a gametophyte sterile type, Yuetai A does not contain such a GTTGAG sequence in the rps16 intron and has a unique G at position 595, which may works as a molecular marker distinguishing the sporophyte sterile type from the gametophyte sterile type. Based on the observation that CMS rice has much lower cytoplasmic polymorphism than indica, japonica and wild rice, it is concluded that CMS rice lack cytoplasm diversity. Therefore, it is important to introduce new sources of cytoplasm into hybrid rice.

Keywords: gametophyte sterility; molecular marker; open reading frame; Oryza sativa; sporophyte sterility
Subjects: chloroplast; chloroplast DNA polymorphism; male sterility; open reading frame; Oryza sativa; rice

Received: August 5, 2008; Accepted: July 16, 2009; Published: September 1, 2009  Show citation

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Ou, L.-J., Huang, G.-W., Li, W.-J., Kang, G.P., Chen, J.-L., Luan, S., & Chen, L.-B. (2009). Chloroplast DNA polymorphism in different types of cytoplasmic male sterile rice. Biologia plantarum, 53(3), 593-596. doi: 10.1007/s10535-009-0108-x
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