biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:631-636, 2009 | DOI: 10.1007/s10535-009-0115-y

Overexpression of the γ-TMT gene in Codonopsis lanceolata

E. S. Seong1, B. K. Ghimire2, E. J. Goh1, J. D. Lim3, M. J. Kim1, I. M. Chung2, C. Y. Yu1,*
1 Bioherb Research Institute, Kangwon National University, Chunchon, South Korea
2 Department of Applied Life Science, Konkuk University, Seoul, South Korea
3 Department of Herbal Medicine Resource, Kangwon National University, Samcheok, South Korea

A cDNA-encoding γ-tocopherol methyltransferase (γ-TMT) from Arabidopsis thaliana was overexpressed in deoduck (Codonopsis lanceolata L.) to improve the tocopherol composition. Deoduck (T2) containing the γ-TMT transgene was produced by Agrobacterium-mediated transformation. Transgene expression was confirmed by polymerase chain reaction and RNA gel blot analysis. The transgenic plants produced more leaves than control plants. In addition, the transgenic plants showed higher levels of the CSOD, CTRX, CAPX, CNADP +-IDCH, and CSO transcripts and higher SOD-like activity compared with the control plants.

Keywords: antioxidant metabolism; CSOD; deoduck; SOD-like activity
Subjects: Codonopsis lanceolata; polymerase chain reaction (PCR); tocopherol; transgenic plants

Received: April 6, 2008; Accepted: August 15, 2008; Published: December 1, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Seong, E.S., Ghimire, B.K., Goh, E.J., Lim, J.D., Kim, M.J., Chung, I.M., & Yu, C.Y. (2009). Overexpression of the γ-TMT gene in Codonopsis lanceolata. Biologia plantarum, 53(4), 631-636. doi: 10.1007/s10535-009-0115-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Agrawala, G.K., Jwac, N.S., Iwahashid, H., Rakwalb, R.: Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling. - Gene 322: 93-103, 2003. Go to original source...
    2. Borland, A., Elliott, S., Patterson, S.: Are the metabolic components of crassulacean acid metabolism up-regulated in responses to an increase in oxidative burden? - J. exp. Bot. 57: 319-328, 2006. Go to original source...
    3. Brigelius-Flohe, R., Traber, M.G.: Vitamin E: function and metabolism. - FASEB J. 13: 1145-1155, 1999. Go to original source...
    4. Cho, K.S., Jang, J.E., Ryon, C., Kwon, M.: Development of transgenic plant (Codonopsis lanceolata Trautv.) harboring a Bialaphos resistance gene bar. - Korean J. Tissue Cult. 26: 281-287, 1999. Go to original source...
    5. Del Rio, L.A., Sandalio, L.M., Corpas, F.J.: Reactive oxygen species and reactive nitrogen species in peroxisomes. Production, scavenging, and role in cell signaling. - Plant Physiol. 141: 330-335, 2006. Go to original source...
    6. Demurin, Y., Skoric, D., Karlovic, D.: Genetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality. - Plant Breed. 115: 33-36, 1996. Go to original source...
    7. Fryer, M.J.: The antioxidant effects of thylakoid vitamin E (alpha;-tocopherol). - Plant Cell Environ. 15: 381-392, 1992. Go to original source...
    8. Gordon, R.G., Alicia, R.V., Carmen, L.W., McIntosh, L.: Transgenic tobacco (Nicotiana tabacum L.) plants with increased expression levels of mitochondrial NADP+-dependent isocitrate dehydrogenase: evidence implicating this enzyme in the redox activation of the alternative oxidase. - Plant Cell Physiol. 45: 1413-1425, 2004. Go to original source...
    9. Goujon, T., Ferret, V., Mila, I., Pollet, B., Ruel, K., Burlat, V., Joseleau, J.P., Barriere, Y., Lapierre, C., Jouanin, L.: Downregulation of the AtCCR1 gene in Arabidopsis thaliana: effects on phenotype lignins and cell wall degradability. - Planta 217: 218-228, 2003. Go to original source...
    10. Grusak, M.A., DellaPenna, D.: Improving the nutrient composition of plants to enhance human nutrition and health. - Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 133-161, 1999. Go to original source...
    11. Hänsch, R., Lang, C., Rennenberg, H., Mendel, R.R.: Significance of plant sulfite oxidase. - Plant Biol. 9: 589-595, 2007. Go to original source...
    12. Hare, P.D., Cress, W.A., Van Staden, J.: Dissecting the roles of osmolyte accumulation during stress. - Plant Cell Environ. 21: 535-553, 1998. Go to original source...
    13. Igamberdiev, A.U., Hill, R.D.: Nitrate, NO and haemoglobin in plant adaptation to hypoxia: an alternative to classic fermentation pathways. - J. exp. Bot. 55: 2473-2482, 2004. Go to original source...
    14. In, J.G., Lee, B.S., Rho, Y.D., Yu, C.Y., Yang, D.C.: Isolation and characterization of thioredoxin cDNA from Codonopsis lanceolata (S. et Z.) Trautv. - Korean J. med. Crop Sci. 13: 293-297, 2005a.
    15. In, J.G., Lee, B.S., Yi, T.H., Yu, C.Y., Yang, D.C.: Molecular cloning and characterization of type 2 metallothonein cDNA from Codonopsis lanceolata (S. et Z.) Trautv. - Korean J. med. Crop. Sci. 15: 288-292, 2005b.
    16. Jo, C., Jeong, I.Y., Kim, D.S., Son, J.H., An, B.J., Choi, J.S., Byun, M.W.: Comparison of the biological activity between a radiation-processed natural extract and a commercial counterpart for an industrial application. - Food Eng. Progr. 9: 177-181, 2005.
    17. Kholová, J., Sairam, R.K., Meena, R.C., Srivastava, G.C.: Response of maize genotypes to salinity stress in relation to osmolytes and metal-ions contents, oxidative stress and antioxidant enzymes activity. - Biol. Plant. 53: 249-256, 2009. Go to original source...
    18. Kiffin, R., Bandyopadhyay, U., Cuervo, A.M.: Oxidative stress and autophagy. - Antioxidants Redox Sign. 8: 152-162, 2006. Go to original source...
    19. Kim, Y.J., Seo, H.Y., Park, T.I., Baek, S.H., Shin, W.C., Kim, H.S., Kim, J.G., Choi, Y.E., Yun, S.J.: Enhanced biosynthesis of α-tocopherol in transgenic soybean by introducing γ-TMT gene. - J Plant Biotechnol. 7: 1-7, 2005.
    20. Laloi, C., Mestres-Ortega, D., Marco, Y., Meyer, Y., Reichheld, J.P.: The arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor. - Plant Physiol. 134: 1006-1016, 2004. Go to original source...
    21. Lang, C., Pork, J., Wirtz, M., Hell, R., Herschbach, C., Kreuzwieser, J., Rennenberg, H., Mendel, R.R., Hansch, R.: Sulphite oxidase as key enzyme for protecting plants against sulphur dioxide. - Plant Cell Environ. 30: 447-455, 2007. Go to original source...
    22. McKersie, B.D., Hoekstra, F.A., Krieg, L.C.: Differences in the susceptibility of plantmembrane lipids to peroxidation. - Biochim. biophys. Acta 1030: 119-126, 1990. Go to original source...
    23. Min, S.R., Yang, S.G., Liu, J.R., Choi, P.S., Soh, W.Y.: High frequency somatic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata. - Plant Cell Rep. 10: 621-623, 1992. Go to original source...
    24. Møller, I.M.: Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. - Annu. Rev.Plant Physiol. Plant mol. Biol. 52: 561-591, 2001. Go to original source...
    25. Noctor, G.: Metabolic signaling in defence and stress: the central roles of soluble redox couples. - Plant Cell Environ. 29: 409-425, 2006. Go to original source...
    26. Piquemal, J., Lapierre, C., Myton, K., O'Connell, A., Schuch, W., Grima-Pettenati, J., Boudet, A.M.: Downregulation of cinnamoyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. - Plant J. 13: 71-83, 1998. Go to original source...
    27. Rimm, E.B., Stampfer, M.J., Ascherio, A., Giovannucci, E., Colditz, G.A., Willett, W.C.: Vitamin E consumption and the risk of coronary heart disease in men. - N. Engl. J. Med. 328: 1450-1456, 1993. Go to original source...
    28. Rogers, L.A., Campbell, M.M.: The genetic control of lignin deposition during plant growth and development. - New Phytol. 164: 17-30, 2004. Go to original source...
    29. Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K.: Regulation and function of ascorbate peroxidase isoenzymes: - J. exp. Bot. 53: 1305-1319, 2002. Go to original source...
    30. Shin, J.H., Park, M.C.: Introduction of RAG25 gene into Codonopsis lanceolata by Agrobacterium tumefaciens. - Korean J. Plant Tissue Cult. 27: 491-496, 2000.
    31. Shintani, D., DellaPenna, D.: Elevating the vitamin E content of plants through metabolic engineering. - Science 282: 2098-2100, 1998. Go to original source...
    32. Van Eenennaam, A.L., Lincoln, K, Durrett, T.P., Valentin, H.E., Shewmaker, C.K., Thorne, G.M., Jiang, J., Baszis, S.R., Levering, C.K., Aasen, E.D., Hao, M. Stein, J.C., Norris, S.R., Last, R.L.: Engineering vitamin E content: from Arabidopsis mutant to soy oil. - Plant Cell 15: 3007-3019, 2003. Go to original source...
    33. Venkata, S.T., Kim, Y.H., Isabelle, A.K., Randy, D.D., Kim, K.H., Glenn, B.C.: Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ -tocopherol methyltransferase gene. - Plant Cell Rep. 26: 61-70, 2007. Go to original source...
    34. Yi, S.Y., Kim, J.H., Joung, Y.H., Lee, S., Kim, W.T., Yu, S.H., Choi, D.: The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis. - Plant Physiol. 136: 2862-2874, 2004. Go to original source...
    35. Yusuf, M.A., Sarin, N.B.: Antioxidant value addition in human diets: genetic transformation of Brassica juncea with γ-TMT gene for increased α-tocopherol content. - Transgenic Res. 16: 109-113, 2007. Go to original source...

    Return to the content