biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:650-656, 2009 | DOI: 10.1007/s10535-009-0118-8

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

N. Bauer1,*, D. Kiseljak1, S. Jelaska1
1 Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia

The leaves of axenically grown Coleus blumei were inoculated with Agrobacterium rhizogenes strain A4 and hairy root were established. PCR and Southern hybridization analysis confirmed transgenic nature of hairy root clones. Cultures of normal roots, induced by α-naphthaleneacetic acid on leaf explants, and hairy roots were evaluated for growth and rosmarinic acid content. Significantly better growth and up to 2.8 higher amount of rosmarinic acid was detected by HPLC analysis in hairy root clones. Methyl jasmonate stimulated rosmarinic acid accumulation in 6 out of 11 tested clones, while yeast extract induced RA accumulation in two and diminished it in 5 out of 11 tested hairy root clones.

Keywords: Agrobacterium rhizogenes; elicitors; phenolic compounds; phenylalanine ammonia lyase; transformation
Subjects: Agrobacterium rhizogenes; Coleus blumei; methyl jasmonate; phenols; polymerase chain reaction (PCR); rosmarinic acid; Southern blot analysis; transgenic plants; yeast extract

Received: September 30, 2007; Accepted: May 5, 2008; Published: December 1, 2009  Show citation

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Bauer, N., Kiseljak, D., & Jelaska, S. (2009). The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots. Biologia plantarum, 53(4), 650-656. doi: 10.1007/s10535-009-0118-8
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