biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:670-676, 2009 | DOI: 10.1007/s10535-009-0121-0

Effects of ozone on wild type and transgenic tobacco

J. Guo1,2, X. F. Li1, D. M. Qi1, S. Y. Chen1, Z. Q. Li1,*, I. Nijs3, Y. G. Li1, G. S. Liu1,*
1 Institute of Botany, Chinese Academy of Sciences, Beijing, P.R. China
2 Graduate School of the Chinese Academy of Sciences, Beijing, P.R. China
3 Department of Biology, University of Antwerp, Wilrijk, Belgium

Tocopherol cyclase (TC, encoded by gene VTE1) catalyzes the penultimate step of tocopherol synthesis. In this study we used wild type and transgenic tobacco plants overexpressing VTE1 from Arabidopsis to examine the role of tocopherol in ozone sensitivity. Wild type plants responded to an 4-h exposure to 300 nmol mol-1 ozone by severe leaf necrosis while the transgenic lines exhibited limited injury. Compared with the wild type, VTE1-overexpressing plants had lower increase in hydrogen peroxide, malondialdehyde contents and ion leakage, and lower decrease of net photosynthetic rate 48 h following the ozone exposure. Transgenic plants also better maintained the structural integrity of the photosynthetic apparatus.

Keywords: hydrogen peroxide; malondialdehyde; ion leakage; photosynthetic rate; tocopherol; ultrastructure
Subjects: chloroplast; electrolyte leakage; hydrogen peroxide; malondialdehyde; Nicotiana tabacum; ozone; photosynthetic rate; stomatal conductance; tobacco; tocopherol; tocopherol cyclase; transgenic plants; transpiration rate; ultrastructure

Received: October 24, 2008; Accepted: September 20, 2009; Published: December 1, 2009  Show citation

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Guo, J., Li, X.F., Qi, D.M., Chen, S.Y., Li, Z.Q., Nijs, I., Li, Y.G., & Liu, G.S. (2009). Effects of ozone on wild type and transgenic tobacco. Biologia plantarum, 53(4), 670-676. doi: 10.1007/s10535-009-0121-0
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