biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:691-696, 2009 | DOI: 10.1007/s10535-009-0124-x

Antioxidant protection during ageing and senescence in transgenic tobacco with enhanced activity of cytokinin oxidase/dehydrogenase

D. Procházková1,*, N. Wilhelmová1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

We studied changes in physiological parameters of whole leaves and in antioxidant protection of chloroplasts during ageing and senescence of tobacco (Nicotiana tabacum L. cv. Samsun NN) leaves with enhanced cytokinin oxidase/dehydrogenase activity (CKX) or without it (WT). Old leaves of CKX plants maintained higher pigment content and photosystem 2 activity compared to WT leaves of the same age. Chloroplasts of old CKX plants showed better antioxidant capacity represented by higher superoxide dismutase, dehydroascorbate reductase and glutathione reductase activities.

Keywords: oxidative stress; reactive oxygen species, transgenic plants
Subjects: ascorbate peroxidase; carotenoids; chlorophyll a,b; chloroplast; cytokinin oxidase/dehydrogenase; glutathione reductase; Nicotiana tabacum; senescence; superoxide dismutase (SOD); tobacco; transgenic plants

Received: January 14, 2009; Accepted: September 20, 2009; Published: December 1, 2009  Show citation

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Procházková, D., & Wilhelmová, N. (2009). Antioxidant protection during ageing and senescence in transgenic tobacco with enhanced activity of cytokinin oxidase/dehydrogenase. Biologia plantarum, 53(4), 691-696. doi: 10.1007/s10535-009-0124-x
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