biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:697-701, 2009 | DOI: 10.1007/s10535-009-0125-9

Leaf morphology and anatomy of transgenic cucumber lines tolerant to downy mildew

M. Szwacka1,*, T. Tykarska3, A. Wisniewska2, M. Kuras3, H. Bilski4, S. Malepszy1
1 Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, Warsaw, Poland
2 Department of Plant Physiology, Warsaw University of Life Sciences, Warsaw, Poland
3 Department of Plant Morphogenesis, University of Warsaw, Warsaw, Poland
4 The Nencki Institute of Experimental Biology, PAS, Warsaw, Poland

The objective of the present paper was to investigate the reason of increased tolerance to the pathogenic fungus Pseudoperonospora cubensis found in transgenic cucumber (Cucumis sativus L.) lines 210 and 212 bearing 35S:cDNA preprothaumatin II gene construct. The tolerance investigation was accomplished by comparing the morphological and anatomical structure of plant leaves. The results obtained demonstrate that leaves of both lines exhibited some anatomical and morphological characteristics (e.g. wax load and composition, cuticle ultrastructure, ultrastructure of secondary wall, arrangement of mesophylll cells) which may be responsible for enhanced tolerance.

Keywords: Cucumis sativus L.; pathogenesis-related proteins; Pseudoperonospora cubensis; thaumatin II
Subjects: cucumber; Cucumis; downy mildew; fungal pathogens; leaf anatomy; leaf epidermis; leaf intracellulars; leaf parenchyma; Pseudoperonospora cubensis; transgenic plants

Received: November 14, 2007; Accepted: June 8, 2008; Published: December 1, 2009  Show citation

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Szwacka, M., Tykarska, T., Wisniewska, A., Kuras, M., Bilski, H., & Malepszy, S. (2009). Leaf morphology and anatomy of transgenic cucumber lines tolerant to downy mildew. Biologia plantarum, 53(4), 697-701. doi: 10.1007/s10535-009-0125-9
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