biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:702-706, 2009 | DOI: 10.1007/s10535-009-0126-8

Chitinases of Coffea arabica genotypes resistant to orange rust Hemileia vastatrix

L. Guerra-Guimarães1,*, M. C. Silva1, C. Struck2, A. Loureiro1, M. Nicole3, C. J. Rodrigues Jr.1, C. P. P. Ricardo4
1 IICT - Centro de Investigação das Ferrugens do Cafeeiro, Quinta do Marquês, Oeiras, Portugal
2 Faculty of Agricultural and Environmental Sciences, University of Rostock, Rostock, Germany
3 Institut de Recherche pour le Développement, UMR RPB, Montpellier Cedex 5, France
4 Instituto de Tecnologia Química e Biológica, Oeiras, Portugal

Two Coffea arabica - Hemileia vastatrix incompatible interactions (I1: coffee cv. Caturra - rust race VI and I2: coffee cv S4 Agaro - rust race II) and a compatible interaction (coffee cv. Caturra - rust race II) were compared in relation to the infection process and chitinase activity. In the two incompatible interactions the fungus ceased growth in the early infection stages, while in the compatible interaction no fungus growth inhibition was observed. A high constitutive level of chitinase activity was detected in the intercellular fluid of healthy leaves. Upon infection, chitinase isoforms were more abundant in incompatible interactions than in the compatible interaction. Immunodetection showed that class I chitinases are particularly relevant in the incompatible interactions and might participate in the defence response of the coffee plants.

Keywords: immunodetection; coffee-orange rust interactions; intercellular fluid; resistance response
Subjects: chitinase; Coffea arabica; compatible interaction; fungal pathogens; Hemileia vastatrix; incompatible interaction; proteins

Received: July 8, 2007; Accepted: September 5, 2008; Published: December 1, 2009  Show citation

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Guerra-Guimarães, L., Silva, M.C., Struck, C., Loureiro, A., Nicole, M., Rodrigues, C.J., & Ricardo, C.P.P. (2009). Chitinases of Coffea arabica genotypes resistant to orange rust Hemileia vastatrix. Biologia plantarum, 53(4), 702-706. doi: 10.1007/s10535-009-0126-8
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