biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:711-714, 2009 | DOI: 10.1007/s10535-009-0128-6

Analysis of genetic variability in two diploid Musa cultivars using RAPD

P. Padmesh1,*, J. V. Reji2, C. Benadict Paul3, S. Mukunthakumar1, G. Praveen1, S. Seeni2
1 Biotechnology and Bioinformatics Division, Tropical Botanic Garden and Research Institute, Palode, India
2 Department of Biotechnology, Sathyabama University, Chennai, India
3 Department of Biotechnology, College of Biomedical Science, Technology and Research, Sri Ramachandra University, Chennai, India

Twenty accessions of the sparsely cultivated diploid Musa cultivars Matti (AA) and Rasakadali (AB) were subjected to random amplified polymorphic DNA (RAPD) assay. A total of 14 random primers were used for the estimation of interand intracultivar variations. Out of 86 bands generated, 64 were polymorphic (74.4 % polymorphism). The cluster analysis grouped the cultivars into two major clusters: cluster I with 10 accessions of Matti and 2 of Rasakadali and cluster II comprising the remaining 8 accessions of Rasakadali. The coefficient of genetic similarity (GS) was from 0.73 to 0.99, suggesting low level of intercultivar variation. The accessions of Rasakadali with mean GS of 0.89 were genetically more diverse than those of Matti (GS = 0.93).

Keywords: banana; genetic similarity coefficient; UPGMA phenogram
Subjects: banana; genetic diversity; Musa sp.; random amplified polymorphic DNA

Received: April 3, 2007; Accepted: June 8, 2008; Published: December 1, 2009  Show citation

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Padmesh, P., Reji, J.V., Benadict Paul, C., Mukunthakumar, S., Praveen, G., & Seeni, S. (2009). Analysis of genetic variability in two diploid Musa cultivars using RAPD. Biologia plantarum, 53(4), 711-714. doi: 10.1007/s10535-009-0128-6
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