biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:719-722, 2009 | DOI: 10.1007/s10535-009-0130-z

In vitro production of solasodine from Solanum trilobatum

K. Anirudhan1, A. S. Nair1,*
1 Department of Botany, University of Kerala, Thiruvananthapuram, India

Sucrose concentration in the culture medium affected chlorophyll content, trichome development and amount of solasodine in regenerated plantlets of Solanum trilobatum. High chlorophyll content and glandular trichomes were observed in the plants grown on Murashige and Skoog basal medium supplemented with 131.85 mM sucrose. The solasodine was quantified using reverse phase high performance liquid chromatography. The plantlets cultivated on this medium yielded 35.97 mg g-1 (d.m.) solasodine whereas the field plants used as control yielded only 2.32 mg g-1 (d.m.) of solasodine.

Keywords: chlorophyll content; HPLC; in vitro regeneration; trichomes
Subjects: auxins; chlorophyll a,b; cytokinins; in vitro culture, micropropagation; Solanum trilobatum; solasodine; sucrose; trichomes

Received: August 15, 2007; Accepted: May 3, 2008; Published: December 1, 2009  Show citation

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Anirudhan, K., & Nair, A.S. (2009). In vitro production of solasodine from Solanum trilobatum. Biologia plantarum, 53(4), 719-722. doi: 10.1007/s10535-009-0130-z
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