biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:741-744, 2009 | DOI: 10.1007/s10535-009-0135-7

AFLP analysis of somaclonal variations in Eucalyptus globulus

X. Y. Mo1,2, T. Long3, Z. Liu4, H. Lin3, X. Z. Liu1, Y. M. Yang1, H. Y. Zhang1,*
1 Southwest Forestry College, White Dragon Temple, Kunming, Yunnan Province, P.R. China
2 South China Agricultural University, Shipai, Guangzhou, Guangdong Province, P.R. China
3 Leizhou Forestry Bureau, Shuiqi, Guangdong Province, P.R. China
4 Center for Biotechnology and Bioengineering, University of Pittsburgh, Pittsburgh, USA

DNA variations of forty-eight Eucalyptus globulus plants, regenerated by successive culture from seven different explants were assessed by AFLP analysis using 18 primer combinations. At least one variation showed 66.7 % of the analyzed plants, and the numbers of polymorphic bands per plant ranged from 1 to 22. The proportion of polymorphic fragments did not correlate with the numbers of the regenerated plants. However, the more times of successive culture were done the more of polymorphic bands were found within the groups. On average, between 97.39 and 99.88 % of all fragments were shared within the same group. AMOVA analysis showed 39.33 % of the variation was found among the accessions that originated from different calli while 60.67 % was from same calli.

Keywords: blue gum; polymorphic bands; successive cultures
Subjects: amplified fragment length polymorphism (AFLP); Eucalyptus globulus; genetic stability

Received: November 8, 2007; Accepted: June 19, 2008; Published: December 1, 2009  Show citation

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Mo, X.Y., Long, T., Liu, Z., Lin, H., Liu, X.Z., Yang, Y.M., & Zhang, H.Y. (2009). AFLP analysis of somaclonal variations in Eucalyptus globulus. Biologia plantarum, 53(4), 741-744. doi: 10.1007/s10535-009-0135-7
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