biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:769-773, 2009 | DOI: 10.1007/s10535-009-0141-9

High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale

X. G. Dai1, X. P. Shi1, Y. M. Ye1, Q. Fu1, M. Z. Bao1,*
1 Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, P.R. China

A high frequency shoot regeneration system for ornamental kale [Brassica oleracea L. var. acephala (D.C.) Alef.] was firstly established from seedling cotyledon and hypocotyl explants. The ability of cotyledon and hypocotyl to produce adventitious shoots varied depending upon genotype, seedling age and culture medium. The maximum shoot regeneration frequency was obtained when the explants from cv. Nagoya 4-d-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg dm-3 6-benzylaminopurine (BA) and 0.1 mg dm-3 naphthaleneacetic acid (NAA). The frequency of shoot regeneration was 65.0 % for cotyledons, 76.1 % for hypocotyls; and the number of shoots per explant was 4.3 for cotyledons, 8.2 for hypocotyls. Hypocotyl explants were found to be more responsive for regeneration when compared with cotyledons. Among the 4 cultivars tested, Nagoya showed the best shoot regeneration response. The addition of 3.0 mg dm-3 AgNO3 was beneficial to shoot regeneration. Roots were formed on the base of the shoots when cultured on half-strength MS medium.

Keywords: AgNO3; auxin; Brassica oleracea var. acephala; cytokinin; genotype; rooting; shoot regeneration
Subjects: auxins; Brassica oleracea; cytokinins; ex vitro transfer; in vitro culture, direct regeneration; in vitro culture, micropropagation; ornamental kale

Received: December 18, 2008; Accepted: July 20, 2009; Published: December 1, 2009  Show citation

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Dai, X.G., Shi, X.P., Ye, Y.M., Fu, Q., & Bao, M.Z. (2009). High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale. Biologia plantarum, 53(4), 769-773. doi: 10.1007/s10535-009-0141-9
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